In vivo assessment of phagocytic properties of Kupffer cells.
In vivo assessment of phagocytic properties of Kupffer cells.
复制标题
库普弗细胞吞噬特性的体内评估。
DOI：
--
复制
发表时间：
1981
影响因子：
9.3
通讯作者：
L. Feinendegen
中科院分区：
文献类型：
--
作者：
S. Reske;K. Vyska;L. Feinendegen
采用三室分析法研究了Tc-99 m标记的人血清白蛋白微粒（Tc-99 mHSA-MM）在人枯否细胞中的吞噬动力学。这些吞噬细胞中的示踪剂周转率可以用具有两阶段消除相的单指数积累来描述。Tc-99 m HSA-MM动力学的三室分析使我们能够量化枯否细胞中的示踪剂附着、吞噬作用和降解。计算的10名对照受试者的吞噬作用时间过程与小鼠巨噬细胞单层中各种供试品的吞噬作用时间过程相同（1）。此外，在10名患有各种肿瘤的患者中观察到巨噬细胞膜的颗粒周转受损，示踪剂的吞噬率显著降低（p <0.01）。
Three-compartment analysis was used to assess the kinetics of phagocytosis of Tc-99m-labeled human serum albumin microparticles (Tc-99m HSA-MM) in human Kupffer cells in vivo. The tracer turnover in these phagocytic cells could be described by a monoexponential accumulation with a two-stage elimination phase. Three-compartment analysis of the Tc-99m HSA-MM kinetics allowed us to quantify tracer attachment, phagocytosis, and degradation in Kupffer cells. The calculated time course of phagocytosis in ten control subjects proved to be identical to that of phagocytosis of various test substances in mouse macrophage monolayers (1). In addition, an impairment of particle turnover at the macrophage membrane, a significantly diminished (p less than 0.01) phagocytosis rate of the tracer, was observed in ten patients with various tumors.