Expression profiles and potential functions of long non-coding RNA in stable angina pectoris patients from Uyghur population of China
Expression profiles and potential functions of long non-coding RNA in stable angina pectoris patients from Uyghur population of China
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中国维吾尔族稳定型心绞痛患者长链非编码RNA的表达谱及潜在功能
DOI：
10.1042/bsr20190364
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发表时间：
2019
影响因子：
4
通讯作者：
Yang Yi Ning
中科院分区：
文献类型：
--
作者：
Zhou Xin Rong;Song Ning;Luo Jun Yi;Zhai Hui;Li Xiang Mei;Zhao Qian;Liu Fen;Li Xiao Mei;Yang Yi Ning
长链非编码rna （lncRNAs）是一种长度超过200 nt的转录本，与心血管疾病（cvd）有关。为了确定lncrna是否与稳定型心绞痛（SAP）有关，我们分析了维吾尔族人群SAP中lncrna和mrna的全基因组表达谱。通过Agilent微阵列（human lncRNA + mRNA Array V4.0）筛选5对SAP患者和健康对照。采用实时定量聚合酶链反应（qRT-PCR）验证50例SAP和50例对照的lncRNA表达水平。使用R和Bioconductor进行数据分析。共鉴定出1871个上调和231个下调的lncrna在外周血单核细胞（PBMCs）中差异表达。芯片分析结果鉴定出lncrna NR_037652.1、ENST00000607654.1、ENST00000589524.1和uc004bhb。3个，经qRT-PCR证实。在筛选的lncrna中，对其共表达mrna的注释结果显示，与NF-κB信号通路、细胞凋亡和p53信号通路相关最显著，而与胆固醇、钙和冠心病相关的疾病主要显著。我们的研究表明，在SAP患者和匹配的对照组中，lncRNAs集群的表达存在显著差异。这些lncrna可能在SAP的发展中发挥重要作用，可以作为未来SAP治疗的生物标志物和潜在靶点。
Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nt that are involved in cardiovascular diseases (CVDs). To determine whether lncRNAs are involved in stable angina pectoris (SAP), we analysed the expression profile of lncRNAs and mRNAs on a genome-wide scale in SAP of Uyghur population. Five pairs of SAP patients and healthy controls were screened by an Agilent microarray (human lncRNA + mRNA Array V4.0). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the lncRNA expression levels in 50 SAP and 50 controls. Data analyses were performed using R and Bioconductor. A total of 1871 up- and 231 down-regulated lncRNAs were identified to be differentially expressed in the peripheral blood mononuclear cells (PBMCs). Microarray analysis results identified the lncRNAs NR_037652.1, ENST00000607654.1, ENST00000589524.1 and uc004bhb.3, which were confirmed by qRT-PCR. Among screened lncRNAs, the annotation result of their co-expressed mRNAs showed that the most significantly related pathways were the NF-κB signalling pathway, apoptosis and the p53 signalling pathway, while the main significantly related diseases were the cholesterol, calcium and coronary disease. Our study indicated that clusters of lncRNAs were significantly differentially expressed between SAP patients and matched controls. These lncRNAs may play a significant role in SAP development and could serve as biomarkers and potential targets for the future treatment of SAP.