Diagnostic role of circulating free plasma DNA detection in patients with localized prostate cancer
Diagnostic role of circulating free plasma DNA detection in patients with localized prostate cancer
复制标题
DOI：
10.1309/dbpx1mfnddjbw1fl
复制
发表时间：
2008-05-01
影响因子：
3.5
通讯作者：
Fiorentino, Michelangelo
中科院分区：
文献类型：
--
作者：
Altimari, Annalisa;Grigioni, Antonia D'Errico;Fiorentino, Michelangelo
为了分析游离血浆DNA（FPDNA）的潜在诊断相关性，我们招募了64例局限性前列腺癌（CaP）患者。通过实时聚合酶链反应评估64名CaP患者和45名健康男性血液样本中的HTERT基因，对FPDNA进行定量。GSTP I基因的甲基化被用来确认选定病例中FPDNA的肿瘤起源。CaP患者FPDNA的平均SD水平（15.4 +/- 10.9 ng/mL）高于对照组（5.5 +/- 3.5 ng/mL; P <0.05）。001）。采用最佳临界值，本试验的敏感性为80%，特异性为82%，受试者工作特征曲线下面积为0.881。高FPDNA值与病理T3分期显著相关（P = 0.035）。在16例FPDNA和16例组织标本中分别有4例（25%）和15例（94%）发现GSTP 1基因甲基化。FPDNA是CaP早期诊断和监测的候选生物标志物。
To analyze the potential diagnostic relevance of free plasma DNA (FPDNA), we enrolled 64 patients with localized prostate cancer (CaP). FPDNA was quantified by real-time polymerase chain reaction assessment of the HTERT gene in blood samples from 64 patients with CaP and 45 healthy males. Methylation of the GSTP I gene was used to confirm the neoplastic origin of FPDNA in selected cases.The mean SD levels of FPDNA were higher in patients with CaP (15.4 +/- 10.9 ng/mL) than in control subjects (5.5 +/- 3.5 ng/mL; P < . 001). By using, the best cutoff value, the sensitivity of the test was 80%, the specificity was 82%, the area under the receiver operating characteristic curve, 0.881. High FPDNA values were significantly associated with pathologic T3 stage (P =.035). Methylation of the GSTP1 gene was found in 4 (25%) of 16 FPDNA samples and 15 (94%) of 16 tissue samples.Quantification of FPDNA discriminates between patients with CaP and healthy subjects and correlates with pathologic tumor stage. FPDNA is a candidate biomarker for early diagnosis and monitoring of CaP.