Pathway Analysis of Selected Circulating miRNAs in Plasma of Breast Cancer Patients: A Preliminary Study.
Pathway Analysis of Selected Circulating miRNAs in Plasma of Breast Cancer Patients: A Preliminary Study.
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DOI：
10.3390/ijms21197288
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发表时间：
2020-10-02
影响因子：
5.6
通讯作者：
Dankova Z
中科院分区：
文献类型：
--
作者：
Holubekova V;Kolkova Z;Grendar M;Brany D;Dvorska D;Stastny I;Jagelkova M;Zelinova K;Samec M;Liskova A;Laucekova Z;Kudela E;Bobrovska M;Kalman M;Zubor P;Dankova Z
乳腺癌（BC）患者循环中的microRNA对于乳腺癌的早期诊断、治疗和监测具有巨大的潜力。这项初步研究的目的是获得选定的miRNA在BC患者的血浆中的表达谱，该表达谱可以区分BC患者和健康志愿者，并且可能在BC的早期检测中有用。通过预测潜在的miRNA靶点的途径分析来评估显著失调的miRNA。该研究招募了来自65名BC患者和34名健康志愿者的血浆样本。通过实时PCR（qPCR）方法在中试测试中筛选选定的miRNA，并通过geNorm算法选择最合适的参考基因进行标准化。在最后的测试中，我们检测到miR-99 a、miR-130 a、miR-484和miR-1260 a（p < 0.05）在BC患者的血浆中显著上调。京都基因和基因组百科全书（KEGG）通路分析揭示了所有显著失调的miRNA都参与了Hippo和转化生长因子-β（TGF-β）信号通路。我们的研究证实了BC患者血浆中所选循环miRNA的不同谱，并强调了分析过程中的一些关键点。
MicroRNAs in the circulation of breast cancer (BC) patients have great potential for the early diagnosis, treatment and monitoring of breast cancer. The aim of this preliminary study was to obtain the expression profile of selected miRNAs in the plasma of BC patients that could discriminate BC patients from healthy volunteers and may be useful in early detection of BC. Significantly deregulated miRNAs were evaluated by pathway analysis with the prediction of potential miRNA targets. The study enrolled plasma samples from 65 BC patients and 34 healthy volunteers. Selected miRNAs were screened in pilot testing by the real-time PCR (qPCR) method, and the most appropriate reference genes were selected for normalisation by the geNorm algorithm. In the final testing, we detected miR-99a, miR-130a, miR-484 and miR-1260a (p < 0.05) as significantly up-regulated in the plasma of BC patients. Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis revealed that all significantly deregulated miRNAs are involved in the Hippo and Transforming Growth Factor-beta (TGF-beta) signalling pathways. Our study confirmed a different profile of selected circulating miRNAs in the plasma of BC patients with an emphasis on some critical points in the analysis process.
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