Identification and expression of two baculovirus gp37 genes.
Identification and expression of two baculovirus gp37 genes.
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DOI：
10.1099/0022-1317-80-7-1823
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发表时间：
1999-07
期刊：
影响因子：
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通讯作者：
C. Phanis;D. P. Miller;S. Cassar;M. Tristem;S. Thiem;D. O'reilly
中科院分区：
文献类型：
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作者：
C. Phanis;D. P. Miller;S. Cassar;M. Tristem;S. Thiem;D. O'reilly
甘蓝夜蛾和舞毒蛾核多角体病毒（MbMNPV和LdMNPV）的gp 37基因已经被鉴定和表征。这两个基因与其他杆状病毒gp 37基因和昆虫痘病毒fusolin基因相似。系统进化分析表明，杆状病毒gp 37基因和昆虫痘病毒fusolin基因形成两个不同的分支。没有证据表明两组之间最近发生了基因转移。gp 37基因也显示出与细菌纤维素和几丁质结合蛋白基因的遥远相似性，但其意义尚不清楚。MbMNPV和LdMNPV gp 37均从共有杆状病毒晚转录起始位点转录。另外，MbMNPV gp 37从一个假定的早期转录起始位点转录。衣霉素处理MbMNPV感染的细胞证实MbMNPV GP 37是N-糖基化的。共聚焦免疫荧光显微镜显示，该蛋白只位于细胞质中，可能在内质网。
The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and characterized. Both genes were similar to other baculovirus gp37 genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct and well-separated clades. There was no evidence of recent gene transfer between the two groups. The gp37 genes also showed a distant similarity to bacterial cellulose- and chitin-binding protein genes, but the significance of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from consensus baculovirus late transcription start sites. MbMNPV gp37 was additionally transcribed from a putative early transcription start site. Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37 is N-glycosylated. Confocal immunofluorescence microscopy revealed that the protein is located exclusively in the cytoplasm, probably in the endoplasmic reticulum.