Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfaces
Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfaces
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钛合金表面微环境修饰调控间充质干细胞体外分化和体内成骨
DOI：
10.1016/j.biomaterials.2012.01.040
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发表时间：
2012-05-01
期刊：
影响因子：
14
通讯作者：
Sung, K. L. Paul
中科院分区：
文献类型：
--
作者：
Hu, Yan;Cai, Kaiyong;Sung, K. L. Paul
为了模拟骨的细胞外微环境，通过层层组装技术在Ti6 Al 4V表面构建了含有骨形态发生蛋白2（BMP 2）和纤维连接蛋白（FN）的明胶/壳聚糖对生物活性多层结构。通过接触角测量、场发射扫描电子显微镜（FE-SEM）和共聚焦激光扫描显微镜（CLSM）分别证实了多层结构的成功制备。生物活性BMP 2随着多层结构的降解而沿着持续释放。在多层涂层的TC 4基质上生长的MSC显示出显著更高（p < 0.01或p < 0.05）的碱性磷酸酶（ALP）产生水平、矿化和侏儒相关转录因子2（Runx 2）、osterix、骨钙素（OC）、骨桥蛋白（OPN）的基因表达。ALP和胶原1型（科尔）与对照组相比，分别培养7天和21天后。更重要的是，MicroCT分析和组织学观察表明，多层涂层Ti6 Al 4V植入物在体内分别在植入4周和12周后促进了其周围的骨密度和新骨形成。结果表明，生物功能多层膜涂层有利于Ti 6Al 4V的成骨和种植体/骨的结合。因此，本研究提出了一种替代制备生物功能化Ti6 Al 4V基植入物在骨科领域的潜在应用。(C)2012爱思唯尔有限公司保留所有权利。
To mimic the extracellular microenvironment of bone, a bioactive multilayered structure of gelatin/chitosan pair, containing bone morphogenetic protein 2(BMP2) and fibronectin (FN), was constructed onto Ti6Al4V surface via a layer-by-layer assembly technique. The successful fabrication of multilayered structure was confirmed by contact angle measurement, field emission scanning electron microscopy (FE-SEM) and confocal laser scanning microscopy (CLSM), respectively. Bioactive BMP2 released in a sustained manner along with the degradation of multilayered structure. MSCs grown onto the multilayer coated TC4 substrates displayed significantly higher (p < 0.01 or p < 0.05) production levels of alkaline phosphatase (ALP), mineralization and genes expressions of runt related transcription factor 2 (Runx2), osterix, osteocalcin (OC), osteopontin (OPN). ALP and collagen type 1(Coll) compared to the controls after culture for 7 days and 21 days, respectively. More importantly, MicroCT analysis and histological observations demonstrated that the multilayer coated Ti6Al4V implants in vivo promoted the bone density and new bone formation around them after implantation for 4 weeks and 12 weeks, respectively. The results indicated that Ti6Al4V coated with biofunctional multilayers was beneficial for osteogenesis and integration of implant/bone. The study therefore presents an alternative to fabricate bio-functionalized Ti6Al4V-based implants for potential application in orthopedics field. (C) 2012 Elsevier Ltd. All rights reserved.