Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens
Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens
复制标题
将慢病毒载体靶向原始生殖细胞（PGC）：产生转基因鸡的有效策略
DOI：
10.24272/j.issn.2095-8137.2020.032
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发表时间：
2020-04
影响因子：
4.9
通讯作者：
Hu Xiao-Xiang
中科院分区：
文献类型：
--
作者：
Jiang Zi-Qin;Wu Han-Yu;Tian Jing;Li Ning;Hu Xiao-Xiang
禽类转基因研究的最新进展突出了利用慢病毒载体作为工具产生转基因鸡的可能性。然而，低的性腺嵌合率和生殖系传递效率仍然限制了该方法在创建转基因鸡中的广泛使用。在这项研究中，我们实施了一个简单的策略，使用修改后的慢病毒载体靶向鸡原始生殖细胞（PGCs），以产生转基因鸡。用修饰的辛德毕斯病毒包膜蛋白（称为M168）假型化慢病毒载体，并与PGC膜蛋白特异性抗体缀合。我们证明了这些优化的M168假型慢病毒载体与SSEA 4抗体缀合，成功地在体外和体内靶向PGC的转导。与对照组相比，M168假型慢病毒转导的鸡胚性腺表达绿色荧光蛋白（GFP）的比例为50.0%~ 66.7%。这将靶向转导效率提高了30.0%-46.7%。还观察到外源基因的有效嵌合。这种靶向技术可以提高种系传播的效率，为转基因家禽的研究提供更多的机会。
Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. In this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells (PGCs) to generate transgenic chickens. The lentiviral vectors were pseudotyped with a modified Sindbis virus envelope protein (termed M168) and conjugated with an antibody specific to PGC membrane proteins. We demonstrated that these optimized M168-pseudotyped lentiviral vectors conjugated with SSEA4 antibodies successfully targeted transduction of PGCs in vitro and in vivo. Compared with the control, 50.0%–66.7% of chicken embryos expressed green fluorescent protein (GFP) in gonads transduced by the M168-pseudotyped lentivirus. This improved the targeted transduction efficiency by 30.0%–46.7%. Efficient chimerism of exogenous genes was also observed. This targeting technology could improve the efficiency of germ line transmission and provide greater opportunities for transgenic poultry studies.
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影响因子：
5.4
作者：
S. Valsesia-Wittmann;F. Morling;B. Nilson;Y. Takeuchi;S. Russell;F. Cosset
通讯作者：
S. Valsesia-Wittmann;F. Morling;B. Nilson;Y. Takeuchi;S. Russell;F. Cosset
影响因子：
3.7
作者：
Wu DT;Seita Y;Zhang X;Lu CW;Roth MJ
通讯作者：
Roth MJ
影响因子：
3.3
作者：
Gruell H;Klein F
通讯作者：
Klein F
涉及生物素化靶向分子的慢病毒载体的多功能靶向系统。
影响因子：
3.7
作者：
Situ K;Chua BA;Bae SY;Meyer AS;Morizono K
通讯作者：
Morizono K
DOI：
10.1242/dev.145367
发表时间：
2017-03-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Taylor L;Carlson DF;Nandi S;Sherman A;Fahrenkrug SC;McGrew MJ
通讯作者：
McGrew MJ