Comparison of the effects of oestrogen deficiency and old age on primary bladder afferent activity and voiding behaviour in the ageing female rat
Comparison of the effects of oestrogen deficiency and old age on primary bladder afferent activity and voiding behaviour in the ageing female rat
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DOI：
10.1111/j.1464-410x.2010.09689.x
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发表时间：
2011-07-01
影响因子：
4.5
通讯作者：
Wyndaele, Jean-Jacques
中科院分区：
文献类型：
--
作者：
Aizawa, Naoki;Iijima, Kazuyoshi;Wyndaele, Jean-Jacques
目的观察雌激素水平降低对去卵巢(OVX)大鼠膀胱传入神经活动的影响，并与老年雌性大鼠进行比较，以确定雌激素(E)水平降低是否会增加膀胱传入神经的活性。每周1次，连续8周。在代谢笼中记录大鼠膀胱单个A-或C-纤维的频率-容量活动。测量后采集血清并测定雌二醇(E_2)水平。结果OVX+V组大鼠C-纤维活性明显低于其他组，而D-纤维活性无明显变化。尽管E_2水平较低，但老龄大鼠仍表现出较高的传入活动和排尿频率。结论E水平低虽然可能影响储存功能，但不是老年雌性大鼠高传入活动和排尿频率的主要原因。
OBJECTIVETo determine if a decrease in oestrogen (E) levels increases activity in bladder afferent nerves, by investigating the effects of E loss on afferent nerve activity in ovariectomized (OVX) rats, and making comparisons with findings in ageing female rats.MATERIALS AND METHODSFemale Sprague-Dawley rats were divided into four groups: ageing; OVX + E; OVX + vehicle (V); and sham-operated (SHAM) + V.The E (250 mu g/kg) or V (cottonseed oil) was injected s.c. once a week for 8 weeks.Frequency-volume recordings were performed with the rats in metabolic cages, and single A delta- or C-fibre activities from the bladder were measured.After measurements, the blood serum was collected and the estradiol (E2) level was measured.RESULTSThe C-fibre activity of OVX + V rats was significantly lower than in the other groups.A delta-fibre activity was unchanged.Despite low E2 levels, ageing rats showed high afferent activity and voiding frequency.CONCLUSIONAlthough a low E level may affect storage function, it is unlikely that it is the main cause of the high afferent activity and voiding frequency observed in the ageing female rat.