Effects of 5-(N-aminohexyl)carbamoyl-2'-deoxyuridine on endonuclease stability and the ability of oligodeoxynucleotide to activate RNase H.
Effects of 5-(N-aminohexyl)carbamoyl-2'-deoxyuridine on endonuclease stability and the ability of oligodeoxynucleotide to activate RNase H.
复制标题
5-(N-氨基己基)氨基甲酰基-2-脱氧尿苷对核酸内切酶稳定性和寡脱氧核苷酸激活 RNase H 能力的影响。
作者：
Y. Ueno;I. Kumagai;N. Haginoya;A. Matsuda
为了评价含5-（N-氨基己基）氨基甲酰基-2 '-脱氧尿苷（Hs）的寡脱氧核苷酸（ODN）的抗核酸内切酶性质，并阐明由ODN类似物及其互补RNA组成的双链体是否诱导RNase H活性，合成了间隔为1、2、3、4和5个天然核苷的含脱氧尿苷类似物Hs的ODN。从用核酸酶S1（一种内切核酸酶）部分水解这些ODNs，发现随着H数目的增加，ODNs对酶的核酸水解变得更稳定。此外，为了检验由含有H的ODN和它们的互补RNA组成的双链体是否是RNase H的底物，用大肠杆菌RNase H处理这些ODN和它们的互补RNA链的双链体。有人发现，裂解的RNA链的酶的动力学影响的双链体中引入的Hs。
To evaluate an endonuclease resistance property of oligodeoxynucleotides (ODNs) containing 5-(N-aminohexyl)carbamoyl-2'-deoxyuridines (Hs) and to elucidate whether a duplex consisting of the ODN analogue and its complementary RNA induces RNase H activity, the ODNs containing the deoxyuridine analogues, Hs, at intervals of one, two, three, four and five natural nucleosides were synthesized. From partial hydrolysis of these ODNs with nuclease S1 (an endonuclease), it was found that the ODNs became more stable towards nucleolytic hydrolysis by the enzyme as the number of H increased. Furthermore, to examine whether the duplexes composed of the ODNs containing Hs and their complementary RNAs are substrates for RNase H or not, the duplexes of these ODNs and their complementary RNA strands were treated with Escherichia coliRNase H. It was found that cleavage of the RNA strands by the enzyme was kinetically affected by the introduction of Hs into the duplexes.
影响因子：
56.9
作者：
YANG, W;HENDRICKSON, WA;SATOW, Y
通讯作者：
SATOW, Y
影响因子：
14.9
作者：
HOKE, GD;DRAPER, K;ECKER, DJ
通讯作者：
ECKER, DJ