Chitosan-Functionalized Graphene Oxide as a Nanocarrier for Drug and Gene Delivery
Chitosan-Functionalized Graphene Oxide as a Nanocarrier for Drug and Gene Delivery
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DOI：
10.1002/smll.201100191
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发表时间：
2011-06-06
期刊：
影响因子：
13.3
通讯作者：
Gan, Leong Huat
中科院分区：
文献类型：
--
作者：
Bao, Hongqian;Pan, Yongzheng;Gan, Leong Huat
氧化石墨烯（GO）与壳聚糖（CS）的共价官能化是通过一个简单的酰胺化过程成功完成的。CS接枝的GO（GO-CS）片由约64重量%的聚乙烯（PE）组成。CS，其赋予它们良好的水溶性和生物相容性。此外，还对GO-CS的理化性质进行了研究。作为一种新型的纳米载体，GO-CS通过π-π堆积和疏水相互作用负载水不溶性抗癌药物喜树碱（CPT）。结果表明，GO-CS对CPT具有上级的负载能力，与纯药物相比，GO-CS-CPT复合物对HepG 2和HeLa细胞具有明显的细胞毒性。同时，GO-CS还能够将质粒DNA浓缩成稳定的纳米级复合物，并且所得GO-CS/pDNA纳米颗粒在一定的氮/磷比例下在HeLa细胞中表现出合理的转染效率。因此，GO-CS纳米载体能够装载和递送抗癌药物和基因。
The covalent functionalization of graphene oxide (GO) with chitosan (CS) is successfully accomplished via a facile amidation process. The CS-grafted GO (GO-CS) sheets consist of about 64 wt.% CS, which imparts them with a good aqueous solubility and biocompatibility. Additionally, the physicochemical properties of GO-CS are studied. As a novel nanocarrier, GO-CS is applied to load a water-insoluble anticancer drug, camptothecin (CPT), via pi-pi stacking and hydrophobic interactions. It is demonstrated that GO-CS possesses a superior loading capacity for CPT, and the GO-CS-CPT complexes show remarkably high cytotoxicity in HepG2 and HeLa cell lines compared to the pure drug. At the same time, GO-CS is also able to condense plasmid DNA into stable, nanosized complexes, and the resulting GO-CS/pDNA nanoparticles exhibit reasonable transfection efficiency in HeLa cells at certain nitrogen/phosphate ratios. Therefore, the GO-CS nanocarrier is able to load and deliver both anticancer drugs and genes.