Molecular cloning, mRNA expression and chromosomal localization of mouse angiotensin-converting enzyme-related carboxypeptidase (mACE2)
Molecular cloning, mRNA expression and chromosomal localization of mouse angiotensin-converting enzyme-related carboxypeptidase (mACE2)
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DOI：
10.1080/1042517021000021608
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发表时间：
2002-08-01
期刊：
影响因子：
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通讯作者：
Hanaoka, K
中科院分区：
文献类型：
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作者：
Komatsu, T;Suzuki, Y;Hanaoka, K
我们分离了两个小鼠cDNA克隆，这些克隆与人血管紧张素转换酶相关的羧肽酶（ACE2）显示出显着的相似性。 cDNA的长度为2746和1995 bp，似乎是通过替代剪接引起的。较长的cDNA编码了798-氨基酸蛋白，该蛋白包含锌金属肽酶之间保守的序列基序。小鼠ACE2与人ACE2的身份显示83％。北印迹分析表明，2.8和2.0-kb的转录本主要在肾脏和肺部表达。将小鼠ACE2基因映射到X染色体X 70.5厘米。
We isolated two mouse cDNA clones which show significant similarities with human angiotensin-converting enzyme-related carboxypeptidase (ACE2). The cDNAs were 2746 and 1995 bp in length and seemed to arise from the same gene by alternative splicing. The longer cDNA encoded a 798-amino acid protein containing the sequence motif conserved among zinc metallopeptidases. Mouse ACE2 showed 83% identity with human ACE2. Northern blot analysis revealed that 2.8- and 2.0-kb transcripts were expressed mainly in the kidney and the lungs. The mouse ACE2 gene was mapped to chromosome x 70.5 cM.