Envelope and long terminal repeat sequences of an infectious murine leukemia virus from a human SCLC cell line: Implications for gene transfer
Envelope and long terminal repeat sequences of an infectious murine leukemia virus from a human SCLC cell line: Implications for gene transfer
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DOI：
10.1023/a:1008020808314
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发表时间：
1998-01-01
期刊：
影响因子：
1.6
通讯作者：
Kiefer, P
中科院分区：
文献类型：
--
作者：
Antoine, M;Wegmann, B;Kiefer, P
开发将基因转移到特定细胞类型或组织中的方法对于实验研究以及临床治疗方法都是重要的。我们在这里报告的包膜（env）基因和U3区的逆转录病毒感染的人小细胞肺癌（SCLC）细胞系的克隆和表征。这种鼠逆转录病毒的复制也完全支持其他肺癌细胞系的不同组织学起源，我们提出的证据表明，长末端重复序列（LTR）-β-半乳糖苷酶（β-Gal）报告构建体以及类似的巨细胞病毒（CMV）启动子P-Gal构建体在人肺上皮细胞系A549和人喉癌细胞系HEp 2。
Development of methods for gene transfer into specific cell types or tissues is important for experimental research as well as clinical therapeutical approaches. We report here the cloning and characterization of the envelope (env) gene and the U3 region of a retrovirus from an infected human Small Cell Lung Cancer (SCLC) cell line. The replication of this murine retrovirus is also fully supported by other lung cancer cell lines of different histological origin, We present evidence that a long terminal repeat (LTR)-beta-galactosidase (beta-Gal) reporter construct performed as well as an analogous cytomegalovirus (CMV) promoter P-Gal construct in the human lung epithelial cell line A549 and in the human larynx carcinoma cell line HEp2.