Multimodal stimulus coding by a gustatory sensory neuron in Drosophila larvae.
Multimodal stimulus coding by a gustatory sensory neuron in Drosophila larvae.
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果蝇幼虫味觉感觉神经元的多模式刺激编码。
DOI：
10.1038/ncomms10687
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发表时间：
2016
影响因子：
16.6
通讯作者：
Sprecher,SimonG
中科院分区：
文献类型：
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作者：
vanGiesen,Lena;Hernandez-Nunez,Luis;Delasoie-Baranek,Sophie;Colombo,Martino;Renaud,Philippe;Bruggmann,Rémy;Benton,Richard;Samuel,AravinthanDT;Sprecher,SimonG
味觉信息的准确感知对于动物的生存至关重要。在成年果蝇中，味觉受体神经元（GRNs）感知与刻板行为反应（如厌恶或吸引）相关的特定味觉模态的化学刺激。我们发现，果蝇的GRNs ofDrosophilalartera采用了一种令人惊讶的不同模式的味觉信息编码。使用一种新的方法钙成像的幼虫味觉系统，我们确定了一个多模式GRN，响应不同的味觉方式与相反的价，如甜蔗糖和苦denatonium，依赖于不同的感觉受体的化学品。这种多模态神经元对于苦味化合物的回避是必不可少的，其人工激活足以介导厌恶。然而，神经元对于味觉混合物的整合也是必不可少的。我们的研究结果支持了幼虫的味觉编码模型，其中不同的受体蛋白介导相同的多模式GRN内的不同反应。
Accurate perception of taste information is crucial for animal survival. In adultDrosophila, gustatory receptor neurons (GRNs) perceive chemical stimuli of one specific gustatory modality associated with a stereotyped behavioural response, such as aversion or attraction. We show that GRNs ofDrosophilalarvae employ a surprisingly different mode of gustatory information coding. Using a novel method for calcium imaging in the larval gustatory system, we identify a multimodal GRN that responds to chemicals of different taste modalities with opposing valence, such as sweet sucrose and bitter denatonium, reliant on different sensory receptors. This multimodal neuron is essential for bitter compound avoidance, and its artificial activation is sufficient to mediate aversion. However, the neuron is also essential for the integration of taste blends. Our findings support a model for taste coding in larvae, in which distinct receptor proteins mediate different responses within the same, multimodal GRN.