Effect of Bakumondo-to on cytochrome P450 activities in rat liver microsomes
Effect of Bakumondo-to on cytochrome P450 activities in rat liver microsomes
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DOI：
10.1016/j.jksus.2016.02.003
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发表时间：
2016-04
期刊：
影响因子：
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通讯作者：
M. Yasuhara;Y. Tayama;Takehiro Kashiwagi;A. Sawa;K. Kihira;K. Miyake
中科院分区：
文献类型：
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作者：
M. Yasuhara;Y. Tayama;Takehiro Kashiwagi;A. Sawa;K. Kihira;K. Miyake
Bakumondo是一种传统的草药。它已被广泛用于治疗慢性气道疾病。最近有报道称几种草药会影响细胞色素P450（CYP）。然而，关于Bakumondo-to对生物活动的影响的信息很少。在本研究中，我们评估了Bakumondo-to对大鼠肝微粒体中CYP活性的影响。大鼠每天两次经口给予Bakumondo-to，剂量为2.0 g/kg体重/天，持续4天。在从给药大鼠中分离的肝微粒体中测定抗氧化活性。使用其特异性底物[分别为7-甲氧基试卤灵、7-甲氧基-4-（三氟甲基）-香豆素和7-苄氧基喹啉]测定CYP 1A 2、CYP 2C和CYP 3A活性。Bakumondo-to使CYP 1A 2活性降低42.5± 7.8%，使CYP 2C活性升高158.0± 29.6%，使CYP 3A活性降低至对照水平的81.5±7.8%。Bakumondo-to诱导CYP 2C活性并降低CYP 1A 2活性;它可能引起药物相互作用。建议在临床环境中应谨慎使用Bakumondo-to和通过CYP 1A 2和CYP 2C代谢的药物的组合。2016作者制作和主办由爱思唯尔BV代表沙特国王大学。这是
Bakumondo-to is a traditional herbal medicine. It has been widely used for the treatment of chronic airway diseases. Recently, it was reported that several herbal medicines affected cytochrome P450 (CYP). However, there is little information about the effects of Bakumondo-to on CYP activities. In this study, we evaluated the effects of Bakumondo-to on CYP activities in rat liver microsomes. Rats were orally treated twice a day with Bakumondo-to at doses of 2.0 g/kg body weight/day for 4 days. CYP activities were determined in liver microsomes isolated from treated rats. CYP1A2, CYP2C, and CYP3A activities were measured using their specific substrates [7-methoxyresorufin, 7-methoxy-4-(trifluoromethyl)-coumarin, and 7-benzyloxyquinoline, respectively]. Bakumondo-to decreased CYP1A2 activity by 42.5±7.8%, increased CYP2C activity by 158.0±29.6%, and decreased CYP3A activity to 81.5±7.8% of the control level. Activities were expressed as percentages of the control.Bakumondo-to induced CYP2C activity and decreased CYP1A2 activity; it may cause drugherbal interactions. It is suggested that combinations of Bakumondo-to and drugs that are metabolized by CYP1A2 and CYP2C should be carefully used in clinical settings. Ó 2016 The Authors. Production and hosting by Elsevier BV on behalf of King Saud University. This is