Distribution of soluble epoxide hydrolase and of cytochrome P4502C8, 2C9, and 2J2 in human tissues
Distribution of soluble epoxide hydrolase and of cytochrome P4502C8, 2C9, and 2J2 in human tissues
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DOI：
10.1177/002215540405200403
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发表时间：
2004-04-01
影响因子：
3.2
通讯作者：
Grant, DF
中科院分区：
文献类型：
--
作者：
Enayetallah, AE;French, RA;Grant, DF
可溶性环氧化物水解酶 (sEH) 可水解多种内源性和外源性环氧化物。许多这些环氧化物被认为是由细胞色素 P450 环氧化酶形成的。在这里，我们通过免疫组织化学报告了 sEH 和细胞色素 P450 环氧化酶 2C8、2C9 和 2J2 的分布。使用高通量组织微阵列评估来自不同器官的大量不同组织。 sEH 存在于肝脏、肾脏和许多其他器官中，包括肾上腺、胰岛、垂体、淋巴组织、肌肉、某些血管平滑肌以及皮肤、前列腺管和胃肠道中的上皮细胞。 sEH 的免疫标记对于特定组织和个体细胞类型具有高度特异性。在几乎所有这些器官和组织中也发现了 CYP2C9，这表明 2C9 和 sEH 在其组织特异性表达模式上非常相似。 CYP2C8 和 2J2 也广泛分布于人体组织中，但与 sEH 相关性较低。结果表明，多种人体组织中内源性脂肪酸环氧化物的产生和水解可能存在不同的途径。
Soluble epoxide hydrolase (sEH) hydrolyzes a wide variety of endogenous and exogenous epoxides. Many of these epoxides are believed to be formed by cytochrome P450 epoxygenases. Here we report the distribution of sEH and cytochrome P450 epoxygenases 2C8, 2C9, and 2J2 by immunohistochemistry. A large number of different tissues from different organs were evaluated using high-throughput tissue microarrays. sEH was found in the liver, kidney, and in many other organs, including adrenals, pancreatic islets, pituitary gland, lymphoid tissues, muscles, certain vascular smooth muscles, and epithelial cells in the skin, prostatic ducts, and the gastrointestinal tract. Immunolabeling for sEH was highly specific for particular tissues and individual cell types. CYP2C9 was also found in almost all of these organs and tissues, suggesting that 2C9 and sEH are very similar in their tissue-specific patterns,of expression. CYP2C8 and 2J2 were also widely distributed in human tissues but were less frequently associated with sEH. The results suggest potentially distinct pathways of endogenous fatty acid epoxide production and hydrolysis in a variety of human tissues.