A window into third-generation sequencing
A window into third-generation sequencing
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DOI：
10.1093/hmg/ddq416
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发表时间：
2010-10-15
影响因子：
3.5
通讯作者：
Kasarskis, Andrew
中科院分区：
文献类型：
--
作者：
Schadt, Eric E.;Turner, Steve;Kasarskis, Andrew
第一代和第二代测序技术引领了基因组学及其他领域的革命，推动了数量惊人的科学进步，包括能够更全面地了解全基因组序列及其编码的信息，更全面地描述甲基化组和转录组，以及更好地理解蛋白质和 DNA 之间的相互作用。尽管如此，基因组生物学的某些测序应用和方面目前超出了当前测序技术的范围，这为该领域的其他创新留下了肥沃的土壤。在这篇综述中，我们描述了填补这一领域的新一代单分子测序技术（第三代测序），它具有显着更长的读取长度、更短的获得结果的时间和更低的总体成本的潜力。
First- and second-generation sequencing technologies have led the way in revolutionizing the field of genomics and beyond, motivating are astonishing number of scientific advances, including enabling a more complete understanding of whole genome sequences and the information encoded therein, a more complete characterization of the methylome and transcriptome and a better understanding of interactions between proteins and DNA. Nevertheless, there are sequencing applications and aspects of genome biology that are presently beyond the reach of current sequencing technologies, leaving fertile ground for additional innovation in this space. In this review, we describe a new generation of single-molecule sequencing technologies (third-generation sequencing) that is emerging to fill this space, with the potential for dramatically longer read lengths, shorter time to result and lower overall cost.