The influence of tissue polarity on nematocyte migration in Hydra attenuata.
The influence of tissue polarity on nematocyte migration in Hydra attenuata.
复制标题
组织极性对细水螅线虫迁移的影响。
DOI：
10.1016/0012-1606(74)90134-1
复制
发表时间：
1974
影响因子：
2.7
通讯作者：
H. Bode
中科院分区：
文献类型：
--
作者：
R. Herlands;H. Bode
研究了水母细胞迁移方向对线虫生长的影响。线状细胞是由体柱中的间质细胞分化而来的，然后高达80%的细胞迁移到触须的外胚层上皮细胞。这些细胞的迁移是明显的顶端定向的，可能是由于趋化性吸引进入后壳和触须，或者是由于体柱组织固有的特性，如再生极性。为了区分这两种可能性，我们比较了未标记的头(下头和触须)在根部或顶端移植到标记体柱上时，~3H-Pro标记的桥丝细胞和狭窄的线虫细胞的积累率。基础移植的头部，如果放置一段适当的时间，就会逆转组织的再生极性。在所有实验中，桥粒迁移的方向与组织再生极性的方向(顶端或底端)相关。此外，通过改变接枝过程或环境条件来改变极性反转的动力学。在每种情况下，Desmoneme迁移逆转的动力学也与组织极性逆转的动力学平行。这些结果表明，组织再生的极性影响着结缔组织的迁移方向。中柱移行在很大程度上不受组织极性的影响，但表现为在化学上被吸引到头部。
Influences underlying the direction of nematocyte migration in hydra were studied. Nematocytes arise by interstitial cell differentiation in the body column, and then up to 80% migrate into the ectodermal epithelial cells of the tentacles. The migration of these cells, which is clearly apically directed, may be due either to a chemotactic attraction into the hypostome and tentacles, or to a property inherent in the tissue of the body column, such as the regeneration polarity. To distinguish between these two possibilities, the rates of accumulation of3H-proline-labeled desmoneme and stenotele nematocytes in unlabeled heads (hypostome and tentacles) grafted either basally or apically to the labeled body column were compared. Basally grafted heads, if left in place for an appropriate length of time, reversed the regeneration polarity of the tissue. In all experiments the direction of desmoneme migration was correlated with the direction (apical or basal) of the regeneration polarity of the tissue. Further, the kinetics of polarity reversal were modified by varying the grafting procedure or the environmental conditions. In every case the kinetics of reversal of desmoneme migration also paralleled the kinetics of reversal of tissue polarity. The results suggest that the direction of desmoneme migration is influenced by the regeneration polarity of the tissue. Stenotele migration was largely unaffected by tissue polarity, but behaved as though chemotactically attracted to the head.