Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs
Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs
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DOI：
10.1126/science.1122689
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发表时间：
2006-04-07
期刊：
影响因子：
56.9
通讯作者：
Schier, AF
中科院分区：
文献类型：
--
作者：
Giraldez, AJ;Mishima, Y;Schier, AF
microRNA（miRNA）占所有脊椎动物基因的1-3％，但它们的体内功能和作用机制仍然很大未知。斑马鱼miR-430在合子转录开始时表达，并在早期发育过程中调节形态发生。通过使用微阵列方法和体内靶验证，我们发现miR-430直接调节了数百个靶标的RNA分子（mRNA）。大多数目标是在没有miR-430的情况下积累的母体表达的mRNA。我们还表明，miR-430加速了靶mRNA的苯基化。这些结果表明，miR-430在早期胚胎发生过程中促进了母体mRNA的去甲基化和清除。
MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.