Structure of an escape mutant of glycoprotein N2 neuraminidase of influenza virus A/Tokyo/3/67 at 3 A.
Structure of an escape mutant of glycoprotein N2 neuraminidase of influenza virus A/Tokyo/3/67 at 3 A.
复制标题
流感病毒 A/Tokyo/3/67 3 A 糖蛋白 N2 神经氨酸酶逃逸突变体的结构。
DOI：
10.1016/0022-2836(88)90344-0
复制
发表时间：
1988
影响因子：
5.6
通讯作者：
Colman,PM
中科院分区：
文献类型：
--
作者：
Varghese,JN;Webster,RG;Laver,WG;Colman,PM
用X射线衍射法测定了流感病毒A/Tokyo/3/67株逃逸突变株的膜糖蛋白神经氨酸酶的三维结构，其分辨率为3？(1？=0.1 nm)。突变病毒是通过在神经氨酸酶的单抗存在下培养病毒而选择的，被证明在残基368处经历了从赖氨酸到谷氨酸的单一氨基酸变化。神经氨酸酶的三维结构与A/Tokyo/3/67报告的结构相同，只是在368位进行了纯粹的局部结构调整。
The three-dimensional structure of the membrane glycoprotein neuraminidase of an escape mutant of the influenza virus strain A/Tokyo/3/67 has been determined to 3 Å (1 Å = 0.1 nm) resolution by X-ray diffraction. The mutant virus, selected by growing the virus in the presence of a monoclonal antibody to the neuraminidase, is shown to have undergone a single amino acid change of lysine to glutamic acid at residue 368. The three-dimensional structure of the neuraminidase is identical with that reported for A/Tokyo/3/67, except for a purely local adjustment of the structure at position 368.