Single-molecule studies of the replisome.
Single-molecule studies of the replisome.
复制标题
DOI：
10.1146/annurev.biophys.093008.131327
复制
发表时间：
2010-05
影响因子：
12.4
通讯作者：
Antoine M. van Oijen;J. Loparo
中科院分区：
文献类型：
--
作者：
Antoine M. van Oijen;J. Loparo
DNA的复制是由复制体完成的，复制体是一个多蛋白复合体，负责解开双亲的DNA并在两条DNA链上合成DNA。复制体复制DNA的速度和过程令人印象深刻，这是复制蛋白质之间一系列严格调控的相互作用的结果。这些蛋白质相互作用的瞬变性质使得用系综平均技术研究复制体的动力学具有挑战性。这篇综述描述了单分子方法，它允许研究单个复制蛋白及其在复制体内的功能。在复制DNA的同时，机械操作单个DNA分子并记录复制体的动态行为的能力使人们对DNA复制的分子机制有了更好的理解。
Replication of DNA is carried out by the replisome, a multiprotein complex responsible for the unwinding of parental DNA and the synthesis of DNA on each of the two DNA strands. The impressive speed and processivity with which the replisome duplicates DNA are a result of a set of tightly regulated interactions between the replication proteins. The transient nature of these protein interactions makes it challenging to study the dynamics of the replisome by ensemble-averaging techniques. This review describes single-molecule methods that allow the study of individual replication proteins and their functioning within the replisome. The ability to mechanically manipulate individual DNA molecules and record the dynamic behavior of the replisome while it duplicates DNA has led to an improved understanding of the molecular mechanisms underlying DNA replication.