Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA
Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA
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DOI：
10.1073/pnas.022638399
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发表时间：
2002-02-19
影响因子：
11.1
通讯作者：
Wang, MD
中科院分区：
文献类型：
--
作者：
Brower-Toland, BD;Smith, CL;Wang, MD
通过使用反馈增强光学陷阱拉伸核小体阵列来检测单个核小体的动态结构。每个核小体的强制分解发生在三个阶段。使用简单的蠕虫状链模型分析数据，在低拉伸力下，组蛋白核心释放了76 bp的DNA。随后，80 bp以更高的力分两个阶段释放：DNA与历史结合的完全延伸，然后是历史的分离。当阵列在达到解离状态之前放松时，核小体能够重新组装并重复拆卸过程。核小体分解的动力学参数也已确定。
The dynamic, structure of individual nucleosomes was examined by stretching nucleosomal arrays with a feedback-enhanced optical trap. Forced disassembly of each nucleosome occurred in three stages. Analysis of the data using a simple worm-like chain model yields 76 bp of DNA released from the histone core at low stretching force. Subsequently, 80 bp are released at higher forces in two stages: full extension of DNA with histories bound, followed by detachment of histories. When arrays were relaxed before the dissociated state was reached, nucleosomes were able to reassemble and to repeat the disassembly process. The kinetic parameters for nucleosome disassembly also have been determined.