Fluorescence Turn‐on of Tetraphenylethylene Derivative by Transfer from Cyclodextrin to Liposomes, HeLa Cells, and E. coli
Fluorescence Turn‐on of Tetraphenylethylene Derivative by Transfer from Cyclodextrin to Liposomes, HeLa Cells, and E. coli
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通过从环糊精转移到脂质体、HeLa 细胞和大肠杆菌来开启四苯乙烯衍生物的荧光
DOI：
10.1002/chem.202203071
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Ikeda Atsushi
中科院分区：
文献类型：
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作者：
Masuda Kosuke;Omokawa Riku;Kawasaki Riku;Mise Yuta;Ooyama Yousuke;Harada Shogo;Shinoda Wataru;Ikeda Atsushi
在此，三甲基-β-环糊精（TMe-β-CDx）和γ-环糊精（γ-CDx）可以通过高速振动研磨将四苯乙烯衍生物（TPE-OH 4）溶解在水中。TMe-β-CDx−TPE− OH 4复合物的荧光强度远高于γ-CDx−TPE− OH 4复合物的荧光强度，因为与γ-CDx空腔相比，光活化后TPE− OH 4中心C=C双键的旋转在较小的TMe-β-CDx空腔中受到抑制。相比之下，γ-CDx−TPE− OH 4复合物的荧光强度非常弱;然而，由于TPE− OH 4从γ-CDx腔转移到脂质膜作为“开启”现象，添加脂质体后荧光强度增加。此外，为了应用温度传感器，证明了脂质体中的荧光强度取决于相变温度。通过使用荧光开启现象，TPE− OH 4可以检测HeLa细胞和E的存在。大肠杆菌荧光。
Herein, trimethyl‐β‐cyclodextrin (TMe‐β‐CDx) and γ‐cyclodextrin (γ‐CDx) could dissolve a tetraphenylethylene derivative (TPE−OH4) in water through high‐speed vibration milling. The fluorescence intensity of the TMe‐β‐CDx−TPE−OH4complex was much higher than that of the γ‐CDx−TPE−OH4complex, as the rotation of the central C=C double bond of TPE−OH4after photoactivation was inhibited in a smaller TMe‐β‐CDx cavity in comparison with the γ‐CDx cavity. In contrast, the fluorescence intensity of the γ‐CDx−TPE−OH4complex was very weak; nevertheless, it increased after the addition of liposomes due to the transfer of TPE−OH4from the γ‐CDx cavity to the lipid membrane as a “turn‐on” phenomenon. Furthermore, to apply temperature sensor, it was demonstrated that the fluorescence intensity in the liposomes depended on the phase‐transition temperature. By using the fluorescence turn‐on phenomenon, TPE−OH4could detect the presence of HeLa cells andE. coliby fluorescence.