Impaired Balance of Th17/Treg in Patients with Nasal Polyposis
Impaired Balance of Th17/Treg in Patients with Nasal Polyposis
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DOI：
10.1111/j.1365-3083.2011.02546.x
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发表时间：
2011-08-01
影响因子：
3.7
通讯作者：
Hong, S. -L.
中科院分区：
文献类型：
--
作者：
Shen, Y.;Tang, X. -Y.;Hong, S. -L.
鼻息肉病（NP）是一种受T淋巴细胞亚群调节的鼻腔和鼻窦慢性炎症性疾病。Th 17/Treg失衡被认为是炎症和特应性反应发展的关键。为了评估NP患者的Th 17/Treg平衡是否被破坏，我们评估了NP特应性患者、NP非特应性患者和对照组外周血单个核细胞（PBMC）中Th 17和Treg细胞的分布。然后，我们测定了三组息肉组织中RORc和Foxp 3的mRNA水平以及IL-17、TGF-β和IL-10的蛋白水平。最后，我们研究了Th 17-、Treg-与Th 1-、Th 2-相关细胞因子（INF-γ、IL-4、IL-5）之间的相关性。结果表明，特应性和非特应性NP患者均显示PBMC中Th 17比例显著增加和Treg比例显著降低，以及息肉组织中RORc和IL-17水平显著增加和Foxp 3和TGF-β水平降低。此外，这些差异在特应性和非特应性组之间是显著的。结果表明，息肉患者外周血单个核细胞中Treg的表达与Th 1、Th 2细胞因子呈负相关。提示NP患者存在Th 17/Treg失衡，特应性疾病患者Treg/Th 17失衡更为严重，提示Treg/Th 17失衡可能在NP的发生发展中起重要作用，特应性疾病可能通过促进Th 17/Treg失衡而加重NP。
Nasal polyposis (NP) is a chronic inflammatory disease of the nasal cavity and sinuses that is regulated by T lymphocyte subsets. Imbalance of Th17/Treg has been considered critical in the development of inflammation and atopic reactions. To assess whether the balance of Th17/Treg is disrupted in patients with NP, we evaluated the distribution of Th17 and Treg cells among peripheral blood mononuclear cells (PBMCs) in atopic patients with NP, non-atopic patients with NP and controls. We then determined mRNA levels of RORc and Foxp3 and protein levels of IL-17, TGF-beta and IL-10 in polyp tissue among the three groups. Finally, we investigated the correlation between Th17-, Treg- and Th1-, Th2-related cytokines (INF-gamma, IL-4, IL-5). The results demonstrated that both atopic and non-atopic patients with NP revealed significantly increased Th17 proportion and decreased Treg proportion in PBMCs, as well as significantly increased RORc and IL-17 levels and decreased Foxp3 and TGF-beta levels in polyp tissue. Furthermore, these differences were significant between atopic and non-atopic groups. The frequency of Treg in PBMCs was found to be negatively correlated with Th1 and Th2 cytokines in polyps. These results indicated that an impaired balance of Th17/Treg existed in patients with NP and was more severe in atopic patients, suggesting that the imbalance of Treg/Th17 may play an important role in the development of NP and that atopy may aggravate NP by promoting the imbalance of Th17/Treg.