Fibronectin-dependent collagen I deposition modulates the cell response to fibronectin
Fibronectin-dependent collagen I deposition modulates the cell response to fibronectin
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DOI：
10.1152/ajpcell.00130.2007
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发表时间：
2007-12-01
影响因子：
5.5
通讯作者：
Chandler, Jennifer
中科院分区：
文献类型：
--
作者：
Sottile, Jane;Shi, Feng;Chandler, Jennifer
纤连蛋白依赖性胶原蛋白 I 沉积调节细胞对纤连蛋白的反应。 Am J Physiol Cell Physiol 293：C1934 - C1946，2007。首次发表于 2007 年 10 月 10 日； doi：10.1152/ajpcell。 00130.2007。 -细胞和细胞外基质 (ECM) 之间的通讯对于细胞生长、存活、迁移和分化的调节至关重要。 ECM 的重塑可以在正常生理条件下（由于组织损伤）和某些病理条件下发生。 ECM 重塑会导致 ECM 组成和组织的改变，从而改变细胞行为的许多方面，包括细胞迁移。细胞迁移反应根据 ECM 分子的类型、数量和组织以及细胞的整合素和蛋白聚糖库而变化。我们和其他人已经证明，包括 I 型和 III 型胶原蛋白在内的多种 ECM 分子的沉积取决于 ECM 纤连蛋白的存在和稳定性。因此，纤连蛋白和纤连蛋白基质对细胞功能的影响可能部分取决于其引导胶原蛋白在 ECM 中沉积的能力。在这项研究中，我们使用胶原蛋白结合纤连蛋白突变体和干扰纤连蛋白-胶原蛋白结合的重组肽来表明，纤连蛋白独立的 I 型胶原蛋白沉积调节细胞对纤连蛋白的迁移反应。这些数据表明，纤连蛋白在 ECM 中组织其他蛋白质的能力是纤连蛋白功能的一个重要方面，并强调了了解 ECM 蛋白质之间的相互作用如何影响细胞行为的重要性。
Fibronectin- dependent collagen I deposition modulates the cell response to fibronectin. Am J Physiol Cell Physiol 293: C1934 - C1946, 2007. First published October 10, 2007; doi: 10.1152/ ajpcell. 00130.2007. -Communication between cells and the extracellular matrix (ECM) is critical for regulation of cell growth, survival, migration, and differentiation. Remodeling of the ECM can occur under normal physiological conditions, as a result of tissue injury, and in certain pathological conditions. ECM remodeling leads to alterations in ECM composition and organization that can alter many aspects of cell behavior, including cell migration. The cell migratory response varies depending on the type, amount, and organization of ECM molecules present, as well as the integrin and proteoglycan repertoire of the cells. We and others have shown that the deposition of several ECM molecules, including collagen types I and III, depends on the presence and stability of ECM fibronectin. Hence, the effect of fibronectin and fibronectin matrix on cell function may partially depend on its ability to direct the deposition of collagen in the ECM. In this study, we used collagen-binding fibronectin mutants and recombinant peptides that interfere with fibronectin-collagen binding to show that fibronect-independent collagen I deposition regulates the cell migratory response to fibronectin. These data show that the ability of fibronectin to organize other proteins in the ECM is an important aspect of fibronectin function and highlight the importance of understanding how interactions between ECM proteins influence cell behavior.