Scaffolds with high oxygen content support osteogenic cell survival under hypoxia
Scaffolds with high oxygen content support osteogenic cell survival under hypoxia
复制标题
高氧含量支架支持成骨细胞在缺氧条件下存活
DOI：
10.1039/d3bm00650f
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发表时间：
2023-07-04
影响因子：
6.6
通讯作者：
Camci-Unal, Gulden
中科院分区：
文献类型：
--
作者：
Augustine, Robin;Camci-Unal, Gulden
大面积骨缺损的再生是一个重大的临床挑战，其成功率参差不齐，但组织工程策略有望实现快速有效的骨再生。维持植入支架内足够的氧气水平是骨组织工程的主要障碍。我们开发了一种新的产氧支架通过静电纺丝聚己内酯与过氧化钙（CaO2）纳米立方体（CPNCs），其特征在于所得到的复合材料的物理，化学和生物学特性。我们的支架是高度多孔的，由包括CPNC的亚微米纤维组成，经XRD和FTIR分析证实。含有CPNC的支架提供了14天的受控氧释放，并支持细胞增殖，同时保护前成骨细胞免受缺氧诱导的细胞死亡。产氧支架在体外也促进了骨模拟缺损的收缩。结果表明，我们的方法可用于开发针对骨缺损的组织工程产品。
Regeneration of large bone defects is a significant clinical challenge with variable success, but tissue engineering strategies are promising for rapid and effective bone regeneration. Maintaining an adequate oxygen level within implanted scaffolds is a major obstacle in bone tissue engineering. We developed a new oxygen-generating scaffold by electrospinning polycaprolactone with calcium peroxide (CaO2) nanocuboids (CPNCs) and characterized the physical, chemical, and biological properties of the resulting composites. Our scaffolds are highly porous and composed of submicron fibers that include CPNC as confirmed with XRD and FTIR analyses. Scaffolds containing CPNC provided controlled oxygen release for 14-days and supported cell proliferation while protecting preosteoblasts from hypoxia-induced cell death. Oxygen-generating scaffolds also facilitated bone mimetic defect contraction in vitro. The results suggest that our approach can be used to develop tissue-engineered products which target bone defects.