Detection of Successful and Unsuccessful Pregnancies in Mice within Hours of Pairing through Frequency Analysis of High Temporal Resolution Core Body Temperature Data.
Detection of Successful and Unsuccessful Pregnancies in Mice within Hours of Pairing through Frequency Analysis of High Temporal Resolution Core Body Temperature Data.
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DOI：
10.1371/journal.pone.0160127
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Kriegsfeld LJ
中科院分区：
文献类型：
--
作者：
Smarr BL;Zucker I;Kriegsfeld LJ
许多可控因素会对胎儿发育产生负面影响，这凸显了早期妊娠检测和识别可靠预测潜在并发症的事件的重要性。临床上，核心体温（CBT）用于帮助计划生育和妊娠检测。然而，此类温度数据通常是通过单次每日测量来收集的。在动物研究中，在胎儿发育的特定阶段进行干预或细胞/组织收获是艰巨的，需要训练有素的观察者定时交配。连续温度测量的价值在很大程度上尚未被探索，但小型、廉价且日益普遍的精确传感器设备的出现使得连续测量变得可行。在这里，我们使用小鼠模型表明，连续、1 分钟分辨率的 CBT 测量可以可靠地实现最早、最准确的妊娠检测（100%，初始配对后 14 小时内），而无需与动物交互来收集数据。这种方法还揭示了一部分雌性在配对后表现出类似怀孕的反应，这种反应持续了不同的天数。小波分析的应用允许在保持时间分辨率的同时进行频率分析，揭示了 CBT 超电频率的显着差异；与明显怀孕失败的情况相比，足月妊娠配对后 12 小时内，这些节律显着增加。高时间分辨率 CBT 和小波分析可以显着早期检测和分离成功妊娠和妊娠样事件。
Many controllable factors negatively impact fetal development, underscoring the importance of early pregnancy detection and identification of events that reliably predict potential complications. Clinically, core body temperature (CBT) is used to aid family planning and pregnancy detection. However, such temperature data typically are gathered in single, daily measurements. In animal studies, interventions or cell/tissue harvesting at defined stages of fetal development are arduous, requiring timed mating by trained observers. The value of continuous temperature measurements remains largely unexplored, but the advent of small, inexpensive, and increasingly ubiquitous, accurate sensor devices makes continuous measures feasible. Here, using a mouse model, we show that continuous, 1-min resolution CBT measurements reliably allow for the earliest and most accurate detection of pregnancy (100%, within 14 h of initial pairing), without requiring interaction with the animal for data collection. This method also reveals a subset of females that exhibit a pregnancy-like response following pairing that persists for a variable number of days. Application of wavelet analysis that permits frequency analysis while preserving temporal resolution, uncovers significant differences in ultradian frequencies of CBT; these rhythms are significantly increased in the 12 h after the day of pairing for pregnancies carried to term compared to apparent pregnancies that failed. High temporal resolution CBT and wavelet analysis permit strikingly early detection and separation of successful pregnancies and pregnancy-like events.