In situ localisation of beet necrotic yellow vein virus (BNYVV) in rootlets of susceptible and resistant beet plants
In situ localisation of beet necrotic yellow vein virus (BNYVV) in rootlets of susceptible and resistant beet plants
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甜菜坏死黄脉病毒（BNYVV）在敏感和抗性甜菜植物根中的原位定位
DOI：
10.1007/bf01321063
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发表时间：
2005
影响因子：
2.7
通讯作者：
R. Goldbach
中科院分区：
文献类型：
--
作者：
O. Scholten;H. Paul;D. Peters;J. Lent;R. Goldbach
通过比较甜菜坏死黄脉病毒(BNYVV)在部分甜菜种质根组织中的增殖和分布情况，研究其对甜菜坏死黄脉病毒(BNYVV)的抗性机制。幼苗要么被含有 BNYVV 的甜菜多粘菌静息孢子的土壤感染，要么被带病毒的游动孢子悬浮液感染。通过这两种接种方法，在易感品种“Regina”的根中获得了高病毒浓度。使用受感染的土壤，在部分抗性品种“Rima”以及抗性材料 Holly 和 WB42 中发现病毒浓度较低。当使用游动孢子悬浮液时，‘Rima’和Holly中出现了与‘Regina’中类似的病毒浓度，而在WB42中发现了较低的病毒浓度。通过原位定位研究，使用免疫金银标记，在被土壤或游动孢子悬浮液感染后，在‘Regina’中检测到病毒，但只能在被游动孢子悬浮液感染后的抗性种质中检测到病毒。在‘Regina’、‘Rima’和Holly的根部，病毒存在于表皮、皮质薄壁组织、内皮层和间质薄壁组织中，但一般不在维管组织内。在 WB42 中，病毒以小聚集体的形式出现，似乎仅限于表皮和一些皮质薄壁细胞。通过比较BNYVV在所研究材料的根中的增殖和分布，可以得出结论：‘Rima’和Holly的病毒抗性机制与WB42不同。
Mechanisms of resistance to beet necrotic yellow vein virus (BNYVV) were studied by comparing the multiplication and distribution of BNYVV in root tissue of some beet accessions. Seedlings were infected either by soil containing resting spores ofPolymyxa betaewith BNYVV, or by a viruliferous zoospore suspension. With both inoculation methods high virus concentrations were obtained in rootlets of the susceptible cultivar ‘Regina’. Using infested soil, low virus concentrations were found in the partially resistant cultivar ‘Rima’ and in the resistant accessions Holly and WB42. When a zoospore suspension was used, similar virus concentrations occurred in ‘Rima’ and Holly as in ‘Regina’, while a low virus concentration was found in WB42. By in situ localisation studies, using immunogold-silver labelling, virus was detected in ‘Regina’ after infection by soil or a zoospore suspension, but it could only be detected in the resistant accessions after infection by a zoospore suspension. In rootlets of ‘Regina’, ‘Rima’ and Holly, virus was found in the epidermis, cortex parenchyma, endodermis, and interstitial parenchyma, but in general not inside the vascular tissue. In WB42 the virus, occurring in small aggregates, seemed to be restricted to the epidermis and some cortex parenchyma cells. Comparing both the multiplication and distribution of BNYVV in rootlets of the accessions studied, it is concluded that the virus resistance mechanism in ‘Rima’ and Holly is different from that in WB42.