Electrochemical Preparation of Photosystem I-Polyaniline Composite Films for Biohybrid Solar Energy Conversion
Electrochemical Preparation of Photosystem I-Polyaniline Composite Films for Biohybrid Solar Energy Conversion
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DOI：
10.1021/acsami.5b01065
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发表时间：
2015-05-13
影响因子：
9.5
通讯作者：
Cliffel, David E.
中科院分区：
文献类型：
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作者：
Gizzie, Evan A.;LeBlanc, Gabriel;Cliffel, David E.
在这项工作中，我们首次报道了通过电化学共聚将生物分子超复合物光系统I（PSI）包埋在聚苯胺导电聚合物网络中。采用恒电位电聚合法在金电极上制备了蛋白质-聚合物复合膜。溶液，含有苯胺和PSI。本研究表明，大膜蛋白质的可控整合到快速制备的复合膜，通过光电化学分析观察到这种蛋白质的截留PSI的独特功能，作为一个高效的生物分子光电二极管产生显着增强的光电流产生的PSI负载聚苯胺膜，相比原始的聚苯胺膜和滴铸PSI膜。然后进行综合研究以分别评估初始聚合溶液中的膜厚度和PSI浓度及其对该新型材料的净光电流的影响。在聚合溶液中用0.1 μ M PSI制备性能最好的复合膜，并沉积到1285 μ m的膜厚，导致平均光电流密度为5.7 μ A cm（-2），效率为0.005%。该光电流输出表示比裸聚苯胺膜增强大于2倍，比具有可比厚度的传统PSI多层膜增强200倍。
In this work, we report for the first time the entrapment Of the biomolecular supercomplex Photosystem I (PSI) within a conductive polymer network of polyaniline via electrochemical copolymerization. Composite polymer-protein films were prepared on gold electrodes through potentiostatic electropolymerization from a single aqueous. solution, containing both aniline and PSI. This study demonstrates the controllable integration of large membrane proteins into rapidly prepared composite films, the entrapment of such proteins was observed through photoelectrochemical analysis PSI's unique function as a highly efficient biomolecular photodiode generated a significant enhancement in photocurrent generation for the PSI loaded polyaniline films, compared to pristine polyaniline films and dropcast PSI films. A comprehensive study was then performed to separately evaluate film thickness and PSI concentration in the initial polymerization solution and their effects on the net photocurrent of this, novel Material. The best performing composite films were prepared with 0.1 mu M PSI in the polymerization solution and deposited to a film thickness of 1285 nth, resulting in an average photocurrent density of 5.7 mu A cm(-2) with an efficiency of 0.005%. This photocurrent output represents an enhancement greater than 2-fold over bare polyaniline films and 200-fold over a traditional PSI multilayer film of comparable thickness.