NMR ''diffusion-diffraction'' of water revealing alignment of erythrocytes in a magnetic field and their dimensions and membrane transport characteristics
NMR ''diffusion-diffraction'' of water revealing alignment of erythrocytes in a magnetic field and their dimensions and membrane transport characteristics
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DOI：
10.1002/mrm.1910370502
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发表时间：
1997-05-01
影响因子：
3.3
通讯作者：
Stilbs, P
中科院分区：
文献类型：
--
作者：
Kuchel, PW;Coy, A;Stilbs, P
对水的“扩散-衍射”实验，产生“q空间”图，对含氧的悬浮液进行（i）这些悬浮液显示出水的扩散-衍射;（ii）q-空间图的形状取决于测量扩散的沿着方向，因此意味着细胞在NMR光谱仪的磁场中对齐;（iii）通过将血红蛋白转化为顺磁形式，以可预测的方式改变扩散各向异性;（iv）通过抑制水传输，以可预测的方式改变q空间图的形状;（v）测量表征对氯汞苯磺酸盐（p-CMBS）对水传输的共价抑制的伪一级速率常数;和（vi）从q空间图中干扰最小值和最大值的位置测量细胞直径和细胞间距。注意到这些发现与基于NMR的组织学表征的相关性，以及对于磁共振成像（MRI）的影响，特别是脑小血管中红细胞排列。
''Diffusion-diffraction'' experiments on water, yielding ''q-space'' plots, were conducted on suspensions of oxygenated (diamagnetic) human erythrocytes, (i) These suspensions displayed diffusion-diffraction of water; (ii) the shape of the q-space plots depended on the direction along which the diffusion was measured, thus implying alignment of the cells in the magnetic field of the NMR spectrometer; (iii) the diffusion anisotropy was altered in a predictable way by converting the hemoglobin to a paramagnetic form; (iv) the shapes of the q-space plots were altered in a predictable way by inhibiting water transport; (v) the pseudo-first order rate constant characterizing the covalent inhibition of water transport, by p-chloromercuribenzenesulfonate (p-CMBS), was measured; and (vi) the cell diameter and intercellular spacing were measured from the positions of the interference minima and maxima in the q-space plots, The relevance of these findings to NMR-based histological characterization of tissues, and the implications, for magnetic resonance imaging (MRI), of erythrocyte alignment in the small vessels of the brain in particular, are noted.