Tubular-wire dual electrode for detection of thiols and disulfides by capillary electrophoresis/electrochemistry.
Tubular-wire dual electrode for detection of thiols and disulfides by capillary electrophoresis/electrochemistry.
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DOI：
10.1021/ac9806198
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发表时间：
1999
影响因子：
7.4
通讯作者：
M. Zhong;S. Lunte
中科院分区：
文献类型：
--
作者：
M. Zhong;S. Lunte
描述了一种用于毛细管电泳的新型双电极检测器。该检测器由作为发生电极的集成金管电极和用于检测的金丝电极组成。检测器配置（包括电极尺寸和位置）在检测灵敏度和分离效率方面进行了优化。双电极与汞合并后，采用毛细管电泳/电化学系统同时检测硫醇和二硫化物。发现胱氨酸的响应在 1 µM 至 1 mM 范围内呈线性，LOD 为 0.5 µM (S/N = 3)，灵敏度为 60 pA/microM。检测限比之前报道的二硫化物检测双电极设计提高了 200 倍。通过核糖核酸酶 A 的胰蛋白酶消化证明了该检测器用于鉴定含硫醇和二硫键的肽的用途。
A new dual-electrode detector for capillary electrophoresis is described. The detector consists of an integrated gold tubular electrode as the generator electrode and a gold wire electrode for detection. The detector configuration, including electrode size and position, has been optimized in terms of detection sensitivity and separation efficiency. After amalgamation of the dual electrode with mercury, the capillary electrophoresis/electrochemistry system was employed for simultaneous detection of thiols and disulfides. The response of cystine was found to be linear from 1 microM to 1 mM with a LOD of 0.5 microM (S/N = 3) and sensitivity of 60 pA/microM. The detection limits represent 200-fold improvement over previously reported dual-electrode designs for the detection of disulfides. The use of this detector for identification of thiol- and disulfide-containing peptides was demonstrated with a tryptic digest of ribonuclease A.