PURIFICATION AND AMINO-ACID-COMPOSITION OF TYPE-A BOTULINUM NEUROTOXIN
PURIFICATION AND AMINO-ACID-COMPOSITION OF TYPE-A BOTULINUM NEUROTOXIN
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DOI：
10.1016/0041-0101(84)90085-0
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发表时间：
1984-01-01
期刊：
影响因子：
2.8
通讯作者：
SATHYAMOORTHY, V
中科院分区：
文献类型：
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作者：
DASGUPTA, BR;SATHYAMOORTHY, V
报道了一种从64l细菌培养物中纯化A型肉毒杆菌神经毒素的方法。程序包括pH 7.0的阳离子交换色谱。最终产品，基本上是同质的（根据聚丙烯酰胺凝胶-十二烷基硫酸钠电泳），是两种形式的神经毒素的混合物（MW 145,000）；二链或有缺口形式（超过95%）及其前体单链或无缺口形式。用本方法纯化的两批神经毒素和用Sugii和Skaguchi方法纯化的1批神经毒素在纯度和氨基酸组成上相似。每个神经毒素分子氨基酸残基数的最佳估计（MW 145,000）为：Asp200Thr75Ser79Glu114Pro44Gly64Ala53Val70CyS10Met22Ile111Leu104Tyr71Phe68Lys100His14Arg43Trp17。
A method to purify type A botulinum neurotoxin from a 64 l bacterial culture is reported. The procedure includes cation exchange chromatography at pH 7.0. the final product, essentially homogeneous (according to polyacrylamide gel-sodium dodecyl sulfate electrophoresis), is a mixture of 2 forms of the neurotoxin (MW 145,000); the dichain or nicked form (over 95%) and its precursor the single chain or unnicked form. Two batches of the neurotoxin purified by the method described here and 1 batch purified according to the method of Sugii and Skaguchi were similar in purity and amino acid composition. The best estimate of the number of amino acid residues per neurotoxin molecule (MW 145,000) is: Asp200Thr75Ser79Glu114Pro44Gly64Ala53Val70CyS10Met22Ile111Leu104Tyr71Phe68Lys100His14Arg43Trp17.