Depletion of dendritic cells, but not macrophages, in patients with sepsis
Depletion of dendritic cells, but not macrophages, in patients with sepsis
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DOI：
10.4049/jimmunol.168.5.2493
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发表时间：
2002-03-01
影响因子：
4.4
通讯作者：
Karl, IE
中科院分区：
文献类型：
--
作者：
Hotchkiss, RS;Tinsley, KW;Karl, IE
树突状细胞（dc）是一组apc，具有与T细胞和B细胞相互作用并调节其对入侵病原体的反应的非凡能力。尽管在脓毒症中发现了许多免疫系统缺陷，但很少有研究检测脓毒症对DCs的影响，这也是本研究的目的。此外，本研究还研究了脓毒症对巨噬细胞的影响，据报道，巨噬细胞会发生凋亡，而MHC II的表达在脓毒症中会降低。对26例败血症患者和20例外伤患者的脾脏进行免疫组化染色。虽然脓毒症并没有减少巨噬细胞的数量，但脓毒症确实导致了fdc占据脾脏面积百分比的显著减少，创伤和脓毒症患者分别为2.9 +/- 0.4 vs 0.7 +/- 0.2%。与创伤患者相比，败血症患者的MHC ii表达细胞（包括交叉指状dc）数量减少。然而，脓毒症似乎并没有诱导那些仍然存在的B细胞、巨噬细胞或dc中MHC II表达的丧失。脓毒症中dc的显著丧失可能显著损害B细胞和T细胞功能，并导致免疫抑制，这是该疾病的一个标志。
Dendritic cells (DCs) are a group of APCs that have an extraordinary capacity to interact with T and B cells and modulate their responses to invading pathogens. Although a number of defects in the immune system have been identified in sepsis, few studies have examined the effect of sepsis on DCs, which is the purpose of this study. In addition, this study investigated the effect of sepsis on macrophages, which are reported to undergo apoptosis, and MHC II expression, which has been noted to be decreased in sepsis. Spleens from 26 septic patients and 20 trauma patients were evaluated by immunohistochemical staining. Although sepsis did not decrease the number of macrophages, sepsis did cause a dramatic reduction in the percentage area of spleen occupied by FDCs, i.e., 2.9 +/- 0.4 vs 0.7 +/- 0.2% in trauma and septic patients, respectively. The number of MHC II-expressing cells, including interdigitating DCs, was decreased in septic, compared with trauma, patients. However, sepsis did not appear to induce a loss of MHC II expression in those B cells, macrophages, or DCs that were still present. The dramatic loss of DCs in sepsis may significantly impair B and T cell function and contribute to the immune suppression that is a hallmark of the disorder.