Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein
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DOI：
10.1074/jbc.271.14.8144
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发表时间：
1996-04-05
影响因子：
4.8
通讯作者：
Furneaux, H
中科院分区：
文献类型：
--
作者：
Ma, WJ;Cheng, S;Furneaux, H
神经元特异性Elav-like蛋白（HuD、Hel-N和HuC）含有三个RNP型共集基序，并与富含AU的元件结合。我们已经鉴定并克隆了这个家族的第四个成员（HuR），它在多种细胞类型中表达。纯化的重组蛋白与c-fos和白细胞介素-3 mRNA中的富含AU的元件紧密结合。在c-fos富含AU的元件的情况下，HuR与含有AUUUA、AUUUUA和AUUUUA基序的27个核苷酸的核心元件结合。突变分析表明，所有三个Au基序都是最大结合所需的。
The neuronal-specific Elav-like proteins (HuD, Hel-N, and HuC) contain three RNP-type concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth member of this family (HuR) that is expressed in a wide variety of cell types. The purified recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs, In the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all three AU motifs are required for maximal binding.