Identification of New Compounds from Sage Flowers (Salvia officinalis L.) as Markers for Quality Control and the Influence of the Manufacturing Technology on the Chemical Composition and Antibacterial Activity of Sage Flower Extracts
Identification of New Compounds from Sage Flowers (Salvia officinalis L.) as Markers for Quality Control and the Influence of the Manufacturing Technology on the Chemical Composition and Antibacterial Activity of Sage Flower Extracts
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DOI：
10.1021/acs.jafc.8b00581
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发表时间：
2018-02-28
影响因子：
6.1
通讯作者：
Speer, Karl
中科院分区：
文献类型：
--
作者：
Gericke, Sebastian;Luebken, Tilo;Speer, Karl
鼠尾草的部分品种，如它的花和叶子，目前被用作烹饪草药和一些药物应用。为了区分不同的鼠尾草提取物，有必要对其单独的化学成分进行分析。它们的特征化合物可作为鼠尾草花和叶提取物的鉴别标志或确定生产工艺和储存条件。三-对香豆醇亚精胺仅在花中检测到，本文首次在鼠尾草和薰衣草中检测到。氧化过程的标志是新的化合物丹参醌A和B，它们是由鼠尾草醇暴露于氧气中产生的。建立了咖啡酸乙酯作为乙醇提取溶剂的间接标记物。半制备高效液相色谱分离后，采用LC-QTOF-HRESIMS、LC-MS、NMR、IR、单晶x射线衍射等方法对化合物进行鉴定。此外，鼠尾草花树脂对革兰氏阳性菌和革兰氏阴性菌均表现出良好的体外抗菌活性。
Parts of Salvia species such as its flowers and leaves are currently used as a culinary herb and for some medicinal applications. To distinguish the different sage extracts it is necessary to analyze their individual chemical compositions. Their characteristic compounds might be established as markers to differentiate between sage flowers and leaf extracts or to determine the manufacturing technology and storage conditions. Tri-p-coumaroylspermidine can be detected only in flowers and has been described here for Salvia and Lavandula species for the first time. Markers for oxidation processes are the novel compounds salviquinone A and B, which were generated from carnosol by exposure to oxygen. Caffeic acid ethyl ester was established as an indirect marker for the usage of ethanol as extraction solvent. The compounds were identified by LC-QTOF-HRESIMS, LC-MS, NMR, IR, and single-crystal X-ray diffraction after isolation by semipreparative HPLC. Furthermore, sage flower resin showed interesting antibacterial in vitro activities against Gram-positive and Gram-negative bacteria.