Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration
Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration
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DOI：
10.1038/sj.jcbfm.9600275
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发表时间：
2006-10-01
影响因子：
6.3
通讯作者：
Abe, Koji
中科院分区：
文献类型：
--
作者：
Deguchi, Kentaro;Tsuru, Kanji;Abe, Koji
对于脑组织再生，一旦坏死的脑组织在损伤（例如脑缺血）后形成空腔，用于迁移或移植的干细胞的任何支架与支持性血管生成是重要的。在这项研究中，一种新的多孔明胶-硅氧烷混合物来自明胶和3-（缩水甘油氧基丙基）三甲氧基硅烷的整合作为三维支架植入到大脑皮层的缺陷。植入病灶的多孔混合物在同一部位保持60天，保持脑形状的完整性，并与周围脑组织良好附着。边缘腔的支架被新形成的组织在大脑中，其中新产生的血管内皮细胞，星形胶质细胞，和小胶质细胞被发现与溴脱氧尿苷双阳性，这些细胞的数量呈剂量依赖性增加与碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）。树突也被发现从周围的大脑皮层延伸到新形成的组织，特别是加入bFGF和EGF。本研究表明，一种新的多孔明胶-硅氧烷杂化物植入中枢神经系统病变后具有生物相容性，从而为细胞迁移、血管生成和树突伸长提供了潜在的支架，并具有添加bFGF和EGF的剂量依赖性效应。
For brain tissue regeneration, any scaffold for migrated or transplanted stem cells with supportive angiogenesis is important once necrotic brain tissue has formed a cavity after injury such as cerebral ischemia. In this study, a new porous gelatin-siloxane hybrid derived from the integration of gelatin and 3-(glycidoxypropyl) trimethoxysilane was implanted as a three-dimensional scaffold into a defect of the cerebral cortex. The porous hybrid implanted into the lesion remained at the same site for 60 days, kept integrity of the brain shape, and attached well to the surrounding brain tissues. Marginal cavities of the scaffolds were occupied by newly formed tissue in the brain, where newly produced vascular endothelial, astroglial, and microglial cells were found with bromodeoxyuridine double positivity, and the numbers of those cells were dose-dependently increased with the addition of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Extension of dendrites was also found from the surrounding cerebral cortex to the newly formed tissue, especially with the addition of bFGF and EGF. The present study showed that a new porous gelatinsiloxane hybrid had biocompatibility after implantation into a lesion of the central nervous system, and thus provided a potential scaffold for cell migration, angiogenesis and dendrite elongation with dose-dependent effects of additive bFGF and EGF.