Multiple alpha subunits of guanine nucleotide-binding proteins in Dictyostelium.
Multiple alpha subunits of guanine nucleotide-binding proteins in Dictyostelium.
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盘基网柄菌中鸟嘌呤核苷酸结合蛋白的多个 α 亚基。
DOI：
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发表时间：
1989
影响因子：
11.1
通讯作者：
P. N. Devreotes
中科院分区：
文献类型：
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作者：
M. Pupillo;A. Kumagai;Geoffrey S. Pitt;R. Firtel;P. N. Devreotes
先前的研究结果表明，在盘基网柄藻中，趋化性和几类基因的表达通过细胞表面cAMP受体与鸟嘌呤核苷酸结合蛋白（G蛋白）相互作用来调节。我们现在描述的cDNA编码的两个G α蛋白亚基的Dictyosteoblastoma的克隆和测序。衍生的氨基酸序列显示它们彼此之间以及与来自哺乳动物和酵母的G α蛋白亚基具有45%的同一性。这两种cDNA都与发育过程中差异表达的多种mRNA互补。这一证据和其他地方提出的突变体的分析表明，他们有不同的生理功能。
Previous results have shown that chemotaxis and the expression of several classes of genes in Dictyostelium discoideum are regulated through a cell surface cAMP receptor interacting with guanine nucleotide-binding proteins (G proteins). We now describe cloning and sequencing of cDNAs encoding two G alpha protein subunits from Dictyostelium. The derived amino acid sequences show that they are 45% identical to each other and to G alpha protein subunits from mammals and yeast. Both cDNAs are complementary to multiple mRNAs that are differentially expressed during development. This evidence and analysis of mutants presented elsewhere suggest that they have distinct physiological functions.