The normal acid base status of mice
The normal acid base status of mice
复制标题
DOI：
10.1016/j.resp.2011.11.015
复制
发表时间：
2012-03-15
影响因子：
2.3
通讯作者：
Wang, Tobias
中科院分区：
文献类型：
--
作者：
Iversen, Nina K.;Malte, Hans;Wang, Tobias
啮齿动物模型通常用于各种生理研究，包括酸碱调节。尽管广泛使用特别是转基因小鼠，但很少有人关注这些动物的正常酸碱状态特征，以揭示适当的控制值。此外，一些研究报告了在麻醉动物中获得的血气值。因此，我们决定在体外表征小鼠血液的血液 CO2 结合特征，并表征清醒 BALBc 小鼠的正常酸碱状态。在旋转眼压计中对与各种 P-CO2 水平平衡的全血进行体外 CO2 解离曲线，显示出典型的哺乳动物 pK'（pK' = 7.816 - 0.234 x pH (r = 0.34)）和非碳酸氢盐缓冲能力（16.1 +/- 2.6 slyke）。为了测量动脉酸碱状态，从颈动脉留置导管的未受干扰的小鼠身上采集少量血液样本。在这些动物中，pH 为 7.391 +/- 0.026，血浆 [HCO3-] 18.4 +/- 0.83 mM，P-CO2 30.3 +/- 2.1 mmHg，乳酸浓度为 4.6 +/- 0.7 mM。因此，我们的研究表明，小鼠的动脉 pH 值与其他哺乳动物相似，尽管动脉 P-CO2 往往低于大型哺乳动物。然而，异氟烷麻醉小鼠动脉血的 pH 值显着降低（pH 7.239 +/- 0.021），而血浆 [HCO3-] 为 18.5 +/- 1.4 mM，P-CO2 为 41.9 +/- 2.9 mmHg，乳酸浓度为 4.48 +/- 0.67 mM。此外，我们测量了代谢和通气 (V-E)，以确定通气需求 (V-E/(V-o2))，以回答小型哺乳动物是否倾向于过度通气。因此，我们建议，小鼠酸碱调节的研究应基于留置导管采集的样本，而不是基于对终末麻醉小鼠进行心脏穿刺的样本。(C) 2011 Elsevier B.V. 保留所有权利。
Rodent models are commonly used for various physiological studies including acid-base regulation. Despite the widespread use of especially genetic modified mice, little attention have been made to characterise the normal acid-base status in these animals in order to reveal proper control values. Furthermore, several studies report blood gas values obtained in anaesthetised animals. We, therefore, decided to characterise blood CO2 binding characteristic of mouse blood in vitro and to characterise normal acid-base status in conscious BALBc mice. In vitro CO2 dissociation curves, performed on whole blood equilibrated to various P-CO2 levels in rotating tonometers, revealed a typical mammalian pK' (pK' = 7.816 - 0.234 x pH (r = 0.34)) and a non-bicarbonate buffer capacity (16.1 +/- 2.6 slyke). To measure arterial acid-base status, small blood samples were taken from undisturbed mice with indwelling catheters in the carotid artery. In these animals, pH was 7.391 +/- 0.026, plasma [HCO3-] 18.4 +/- 0.83 mM, P-CO2 30.3 +/- 2.1 mmHg and lactate concentration 4.6 +/- 0.7 mM. Our study, therefore, shows that mice have an arterial pH that resembles other mammals, although arterial P-CO2 tends to be lower than in larger mammals. However, pH from arterial blood sampled from mice anaesthetised with isoflurane was significantly lower (pH 7.239 +/- 0.021), while plasma [HCO3-] was 18.5 +/- 1.4 mM, P-CO2 41.9 +/- 2.9 mmHg and lactate concentration 4.48 +/- 0.67 mM. Furthermore, we measured metabolism and ventilation (V-E) in order to determine the ventilation requirements (V-E/(V-o2) to answer whether small mammals tend to hyperventilate. We recommend, therefore, that studies on acid-base regulation in mice should be based on samples taken for indwelling catheters rather than cardiac puncture of terminally anaesthetised mice. (C) 2011 Elsevier B.V. All rights reserved.