CDC20 and CDH1: A family of substrate-specific activators of APC-dependent proteolysis
CDC20 and CDH1: A family of substrate-specific activators of APC-dependent proteolysis
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DOI：
10.1126/science.278.5337.460
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发表时间：
1997-10-17
期刊：
影响因子：
56.9
通讯作者：
Amon, A
中科院分区：
文献类型：
--
作者：
Visintin, R;Prinz, S;Amon, A
由后期促进复合物 (APC) 介导的蛋白水解会触发染色体分离并退出有丝分裂，但其调控机制尚不清楚。保守的 Cdc20 和 Cdh1 蛋白被鉴定为 APC 依赖性蛋白水解的限制性底物特异性激活剂。 APC 底物 Pds1 的降解需要 CDC20，但其他 APC 底物（如 Clb2 和 Ase1）的降解则不需要。相反，cdh1 Delta 突变体的 Ase1 和 Clb2 降解受到损害，但 Pds1 的降解却没有受到损害。 CDC20 或 CDH1 的过表达足以在底物通常稳定的细胞周期阶段诱导适当靶标的 APC 依赖性蛋白水解。
Proteolysis mediated by the anaphase-promoting complex (APC) triggers chromosome segregation and exit from mitosis, yet its regulation is poorly understood. The conserved Cdc20 and Cdh1 proteins were identified as limiting, substrate-specific activators of APC-dependent proteolysis. CDC20 was required for the degradation of the APC substrate Pds1 but not for that of other APC substrates, such as Clb2 and Ase1. Conversely, cdh1 Delta mutants were impaired in the degradation of Ase1 and Clb2 but not in that of Pds1. Overexpression of either CDC20 or CDH1 was sufficient to induce APC-dependent proteolysis of the appropriate target in stages of the cell cycle in which substrates are normally stable.