araucan and caupolican provide a link between compartment subdivisions and patterning of sensory organs and veins in the Drosophila wing
araucan and caupolican provide a link between compartment subdivisions and patterning of sensory organs and veins in the Drosophila wing
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DOI：
10.1101/gad.10.22.2935
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发表时间：
1996-11-15
影响因子：
10.5
通讯作者：
Modolell, J
中科院分区：
文献类型：
--
作者：
GomezSkarmeta, JL;Modolell, J
同源盒前模式基因arucan(Ara)和caupolica(Cucp)在发育中的果蝇翅膀的前后室(AP)边界附近的两个对称斑块中共同表达，位于背腹侧(DV)室边界的两侧。Ara-caup在这些斑块上的表达对于未来的L3静脉和相关感觉器官的指定是必要的，这是通过在较小的重叠区域中转录激活菱形/微静脉以及无毛和无毛和无毛的神经基因来实现的。我们发现，Ara-Caup在这些斑块的表达是由Hedgehog信号介导的，它通过诱导靠近AP室边界的前部细胞中高水平的Cubitus interruptus(Ci)蛋白；高水平的Ci激活了十五肢瘫痪(DPP)的表达，并且Ci和DPP共同正向控制Ara-Caup。斑块的后缘显然是由Engraded的抑制所定义的。无翼堆积在DV的边界上，也受到压制，两块之间的缝隙。因此，Ara和Caup将影响翼盘初级细分的基因的输入整合到不同的区域中，以确定两个较小的区域。这些反过来又有助于创建更小的菱形/细脉和无毛鳞状表达区域。
The homeo box prepattern genes araucan (ara) and caupolican (caup) are coexpressed near the anterior-posterior (AP) compartment border of the developing Drosophila wing in two symmetrical patches located one at each side of the dorsoventral (DV) compartment border. ara-caup expression at these patches is necessary for the specification of the prospective vein L3 and associated sensory organs through the transcriptional activation, in smaller overlapping domains, of rhomboid/veinlet and the proneural genes achaete and scute. We show that ara-caup expression at those patches is mediated by the Hedgehog signal through its induction of high levels of Cubitus interruptus (Ci) protein in anterior cells near to the AP compartment border;The high levels of Ci activate decapentaplegic (dpp) expression, and, together, Ci and Dpp positively control ara-caup. The posterior border of the patches is apparently defined by repression by engrailed. Wingless accumulation at the DV border sets, also by repression, the gap between the two patches. Thus, ara and caup integrate the inputs of genes effecting the primary subdivisions of the wing disc into compartments to define two smaller territories. These in turn help create the even smaller domains of rhomboid/veinlet and achaete-scute expression.