Widespread monoallelic expression on human autosomes
Widespread monoallelic expression on human autosomes
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DOI：
10.1126/science.1148910
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发表时间：
2007-11-16
期刊：
影响因子：
56.9
通讯作者：
Chess, Andrew
中科院分区：
文献类型：
--
作者：
Gimelbrant, Alexander;Hutchinson, John N.;Chess, Andrew
在母本和父本等位基因之间随机选择的单等位基因表达定义了一类不寻常的基因，包括 X 失活基因和一些常染色体基因家族。使用全基因组方法，我们评估了克隆细胞系中约 4000 个人类基因的等位基因特异性转录，发现超过 300 个基因发生随机单等位基因表达。对于大多数单等位基因，我们还观察到一些显示双等位基因表达的克隆系。克隆细胞系反映了表达这些基因的母本、父本或两个等位基因的独立选择。这可能导致表达的蛋白质序列的差异和基因表达水平的差异。出乎意料地广泛的单等位基因表达表明了一种在个体细胞及其克隆后代中产生多样性的机制。
Monoallelic expression with random choice between the maternal and paternal alleles defines an unusual class of genes comprising X-inactivated genes and a few autosomal gene families. Using a genome-wide approach, we assessed allele-specific transcription of about 4000 human genes in clonal cell lines and found that more than 300 were subject to random monoallelic expression. For a majority of monoallelic genes, we also observed some clonal lines displaying biallelic expression. Clonal cell lines reflect an independent choice to express the maternal, the paternal, or both alleles for each of these genes. This can lead to differences in expressed protein sequence and to differences in levels of gene expression. Unexpectedly widespread monoallelic expression suggests a mechanism that generates diversity in individual cells and their clonal descendants.