Local cortical pulling-force repression switches centrosomal centration and posterior displacement in C-elegans
Local cortical pulling-force repression switches centrosomal centration and posterior displacement in C-elegans
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DOI：
10.1083/jcb.200706005
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发表时间：
2007-12-31
影响因子：
7.8
通讯作者：
Onami, Shuichi
中科院分区：
文献类型：
--
作者：
Kimura, Akatsuki;Onami, Shuichi
中心体的定位是由中心体辐射的微管上的力主动调节的。两种机制，中心导向和极化皮质拉，是主要的贡献者连续的中心和posteriorly位移迁移的中心体在单细胞阶段秀丽隐杆线虫。在这项研究中，我们分析了作用在中心体上的力的空间分布，以研究中心体迁移从居中到移位的机制。我们澄清的空间分布的力量，使用图像处理来测量微米级的运动的中心体。分布的变化表明，极化皮质牵拉功能，在中心迁移。向后定向的极化皮质拉力在居中迁移期间主要在外侧区域受到抑制，并且在向后移位迁移期间被解除抑制。计算机模拟表明，这种皮质拉力的局部抑制足以在定心和移位迁移之间切换。皮质牵拉的局部调节可能是中心体动态定位精确时间调节的一种保守机制。
Centrosome positioning is actively regulated by forces acting on microtubules radiating from the centrosomes. Two mechanisms, center-directed and polarized cortical pulling, are major contributors to the successive centering and posteriorly displacing migrations of the centrosomes in single-cell-stage Caenorhabditis elegans. In this study, we analyze the spatial distribution of the forces acting on the centrosomes to examine the mechanism that switches centrosomal migration from centering to displacing. We clarify the spatial distribution of the forces using image processing to measure the micrometer-scale movements of the centrosomes. The changes in distribution show that polarized cortical pulling functions during centering migration. The polarized cortical pulling force directed posteriorly is repressed predominantly in the lateral regions during centering migration and is de-repressed during posteriorly displacing migration. Computer simulations show that this local repression of cortical pulling force is sufficient for switching between centering and displacing migration. Local regulation of cortical pulling might be a mechanism conserved for the precise temporal regulation of centrosomal dynamic positioning.