Epidermal secretions of terrestrial flatworms and slugs:: Lehmannia valentiana mucus contains matrilin-like proteins
Epidermal secretions of terrestrial flatworms and slugs:: Lehmannia valentiana mucus contains matrilin-like proteins
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DOI：
10.1016/j.cbpb.2007.06.001
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发表时间：
2007-11-01
影响因子：
2.2
通讯作者：
Graham, Lloyd D.
中科院分区：
文献类型：
--
作者：
Li, Dongmei;Graham, Lloyd D.
我们描述了两种类型的陆生无脊椎动物的表皮粘液：自由生活的扁形虫（Tricladida：Terricola），和蛞蝓Lehmannia valentiana（腹足纲：Pulmonata）。两者表现出相似的干剪切强度（1.4-1.7 MPa）。在变性凝胶电泳中，扁形虫粘液的蛋白质组分主要以宽带（200-300 kDa）迁移。鼻涕虫粘液比扁形虫粘液具有更高的蛋白质含量，但其含有比蛋白质更多的碳水化合物，主要为大的硫酸乙酰肝素样糖胺聚糖。蛋白质和糖胺聚糖对于段塞水凝胶的机械完整性都是必不可少的。蛞蝓粘液的蛋白质级分含有类似于12种较大的蛋白质（30-300 kDa）和类似于6种较小的蛋白质（10-28 kDa）。获得蛞蝓粘液40 kDa蛋白（Sm 40; Genbank登录号EF 634345）和85 kDa蛋白（Sm 85; Genbank登录号EF 634346）的完整cDNA克隆。这两种蛋白质都含有EGF样重复序列和von Willebrand A结构域，因此类似于脊椎动物matrilin。许多较大的鼻涕虫粘液蛋白似乎含有A-结构域，这些可能在腹足类粘液的不寻常流变特性中发挥作用。皇冠版权所有（c）2007由爱思唯尔公司出版。All rights reserved.
We describe the epidermal mucus of two types of terrestrial invertebrates: free-living flatworms (Tricladida: Terricola), and the slug Lehmannia valentiana (Gastropoda: Pulmonata). Both exhibited similar dry shear strengths (1.4-1.7 MPa). In denaturing gel electrophoresis, the protein fraction of flatworm mucus rnigrated mainly as a broad band (200-300 kDa). Slug mucus had a higher protein content than flatworm mucus but it contained more carbohydrate than protein, mainly as large heparan sulfate-like glycosaminoglycans. Proteins and glycosaminoglycans were both essential for the mechanical integrity of the slug hydrogel. The protein fraction of slug mucus contained similar to 12 larger proteins (30-300 kDa) and similar to 6 smaller ones (10-28 kDa). Complete cDNA clones were obtained for the slug mucus 40 kDa protein (Sm40; Genbank accession EF634345) and 85 kDa protein (Sm85; Genbank accession EF634346). Both proteins contain EGF-like repeats and von Willebrand A-domains, and therefore resemble vertebrate matrilins. Many of the larger slug mucus proteins appear to contain A-domains, and these may play a role in the unusual rheological properties of gastropod mucus. Crown Copyright (c) 2007 Published by Elsevier Inc. All rights reserved.