DIMERIC STRUCTURE OF SINGLE CHLORIDE CHANNELS FROM TORPEDO ELECTROPLAX
DIMERIC STRUCTURE OF SINGLE CHLORIDE CHANNELS FROM TORPEDO ELECTROPLAX
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DOI：
10.1073/pnas.81.9.2772
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
WHITE, MM
中科院分区：
文献类型：
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作者：
MILLER, C;WHITE, MM
4，4“-二异硫氰基-2，2”-二苯乙烯二磺酸盐（DIDS）对T.研究了掺入平面磷脂双层膜中的California Electroplax。DIDS不可逆且快速地抑制含有许多通道的膜的宏观电导。在单通道水平上，DIDS的影响更为复杂。未抑制的单通道显示电导为20、10和0 pS的3个基板。短时间暴露（5-30秒）于10 μ M DIDS将该3级活性通道转换成10-pS电导的常规通道。更长的曝光时间消除了所有通道波动。Cl-通道被构建为相同蛋白质亚基的功能性二聚体。
The inhibition by 4,4''-diisothiocyano-2,2''-stilbenedisulfonate (DIDS) of Cl- channels from T. californica electroplax incorporated in planar phospholipid bilayer membranes is studied. DIDS irreversibly and rapidly inhibits the macroscopic conductance of membranes containing many channels. At the single-channel level, the effect of DIDS is more complicated. The uninhibited single channel displays 3 substrates of conductances 20, 10, and 0 pS. Short exposure (5-30 s) to 10 .mu.M DIDS converts this 3-level active channel into a conventional channel of 10-pS conductance. Longer exposure eliminates all channel fluctuations. The Cl- channel is constructed as a functional dimer of identical protein subunits.