Systematic stereoscopic analyses for cloacal development: The origin of anorectal malformations.
Systematic stereoscopic analyses for cloacal development: The origin of anorectal malformations.
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DOI：
10.1038/srep13943
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发表时间：
2015-09-10
影响因子：
4.6
通讯作者：
Yamada G
中科院分区：
文献类型：
--
作者：
Matsumaru D;Murashima A;Fukushima J;Senda S;Matsushita S;Nakagata N;Miyajima M;Yamada G
胚胎泄殖腔的分裂是消化道和泌尿道形成的最基本事件。泄殖腔发育缺陷导致肛门直肠畸形（ARMs;每10，000例活产2-5例）。然而，ARMs的发育和致病机制尚不清楚。在目前的研究中，我们可视化的上皮细胞在发展中的泄殖腔和肾导管（ND）。系统的立体分析表明，ND泄殖腔连接网站从泄殖腔的外侧中部背前部的泄殖腔在泄殖腔分裂E10.5至E11.5的小鼠胚胎。遗传细胞标记分析表明，腹泄殖腔上皮细胞在早期阶段很少贡献的背部。此外，我们揭示了可能的内胚层细胞内的前部分的尿生殖窦和后肠的形态发生运动。这些结果为了解泄殖腔发育和ARM发病机制提供了基础。
The division of the embryonic cloaca is the most essential event for the formation of digestive and urinary tracts. The defective development of the cloaca results in anorectal malformations (ARMs; 2–5 per 10,000 live births). However, the developmental and pathogenic mechanisms of ARMs are unclear. In the current study, we visualized the epithelia in the developing cloaca and nephric ducts (NDs). Systemic stereoscopic analyses revealed that the ND-cloaca connection sites shifted from the lateral-middle to dorsal-anterior part of the cloaca during cloacal division from E10.5 to E11.5 in mouse embryos. Genetic cell labeling analyses revealed that the cells in the ventral cloacal epithelium in the early stages rarely contributed to the dorsal part. Moreover, we revealed the possible morphogenetic movement of endodermal cells within the anterior part of the urogenital sinus and hindgut. These results provide the basis for understanding both cloacal development and the ARM pathogenesis.