Ethyl pyruvate enhances ATP levels, reduces oxidative stress and preserves cardiac function in a rat model of off-pump coronary bypass.
Ethyl pyruvate enhances ATP levels, reduces oxidative stress and preserves cardiac function in a rat model of off-pump coronary bypass.
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DOI：
10.1016/j.hlc.2004.12.021
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发表时间：
2005-03-01
期刊：
影响因子：
--
通讯作者：
Woo, Y Joseph
中科院分区：
文献类型：
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作者：
Taylor, Matthew D;Grand, Todd J;Woo, Y Joseph
背景技术背景：非体外循环冠状动脉旁路移植术在血运重建过程中会出现短暂的心肌缺血，导致心肌收缩功能障碍和氧化损伤。本研究的目的是探讨丙酮酸乙酯作为心肌保护剂在非体外循环冠状动脉旁路移植术与短暂性心肌功能不全无infarsion.METHODS：Wistar大鼠模型的疗效通过短暂缺血10分钟闭塞的LAD冠状动脉，然后再灌注10分钟。在缺血和再灌注开始之前，动物立即接受作为对照的林格氏溶液（n=10）或林格氏丙酮酸乙酯（n=10）的IV推注。心肌ATP和脂质过氧化水平进行了定量的能量和氧化应激的估计，分别。结果：丙酮酸乙酯显著增加心肌ATP水平相比，对照组（2650+/-759 nmol/g对892+/-276 nmol/g，p=0.04）。与对照组相比，丙酮酸乙酯处理的动物心肌氧化应激显著降低（70.4+/-2.6 nmol/g vs. 81.8+/-2.4 nmol/g，p=0.04）。dP/dt max和心输出量显着更大的丙酮酸乙酯组相比，对照组在缺血和reperfusion.CONCLUSIONS：丙酮酸乙酯提高心肌ATP水平，降低氧化应激，并保留心肌功能的模型短暂缺血/再灌注损伤不受心肌梗死。
BACKGROUND: Off-pump coronary artery bypass grafting is associated with transient periods of myocardial ischemia during revascularization resulting in myocardial contractile dysfunction and oxidative injury. The purpose of this study was to investigate the efficacy of ethyl pyruvate as a myocardial protective agent in a rat model of off-pump coronary artery bypass grafting associated with transient myocardial dysfunction without infarction.METHODS: Wistar rats were subjected to transient ischemia via 10 min occlusion of the LAD coronary artery followed by 10 min of reperfusion. Animals received an IV bolus of Ringer's solution as a control (n=10) or Ringer's ethyl pyruvate (n=10) immediately before the initiation of ischemia and reperfusion. Myocardial ATP and lipid peroxidation levels were quantified for an estimation of energetics and oxidative stress, respectively. In vivo cardiac function was assessed throughout the ischemia and reperfusion periods.RESULTS: Ethyl pyruvate significantly increased myocardial ATP levels compared to controls (2650+/-759 nmol/g versus 892+/-276 nmol/g, p=0.04). Myocardial oxidative stress was significantly reduced in animals treated with ethyl pyruvate compared to controls (70.4+/-2.6 nmol/g versus 81.8+/-2.4 nmol/g, p=0.04). dP/dt max and cardiac output were significantly greater in the ethyl pyruvate group compared to controls during ischemia and reperfusion.CONCLUSIONS: Ethyl pyruvate enhances myocardial ATP levels, reduces oxidative stress, and preserves myocardial function in a model of transient ischemia/reperfusion injury not subject to myocardial infarction.