Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film
Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film
复制标题
基于三维有序大孔金膜的电化学阻抗免疫传感器
DOI：
10.1021/ac7021376
复制
发表时间：
2008-03-15
影响因子：
7.4
通讯作者：
Zhu, Jun-Jie
中科院分区：
文献类型：
--
作者：
Chen, Xiaojun;Wang, Yuanyuan;Zhu, Jun-Jie
基于三维有序大孔（3DOM）金膜修饰电极，利用电化学阻抗谱（EIS）技术研制了一种新型的c反应蛋白（CRP）无标记免疫传感器。该电极采用倒置蛋白石模板进行电化学制备，使3DOM金膜的表面积比传统裸平面膜的表面积高出14.4倍，并通过循环伏安（CV）技术对其进行表征。由相互连接的金纳米颗粒组成的3DOM金膜不仅具有良好的生物相容性微环境，而且促进了电导率和稳定性的提高。将CRP抗体与3-巯基丙酸（MPA）共价偶联在3DOM金膜电极上，研制出CRP免疫传感器。通过相应的CRP抗原与CRP抗体特异性结合时阻抗值的增加来测定CRP浓度。增加的电子转移电阻（R-et）值与CRP浓度的对数值成正比，在0.1至20 ng mL（-1）范围内。从医院获得的三种血清中检测CRP水平显示出可接受的准确性。
A novel label-free immunosensor for the detection of C-reactive protein (CRP) was developed based on a three-dimensional ordered macroporous (3DOM) gold film modified electrode by using the electrochemical impedance spectroscopy (EIS) technique. The electrode was electrochemically fabricated with an inverted opal template, making the surface area of the 3DOM gold film up to 14.4 times higher than that of a classical bare flat one, characterized by the cyclic voltammetric (CV) technique. The 3DOM gold film which was composed of interconnected gold nanoparticles not only has a good biocompatible microenvironment but also promotes the increase of conductivity and stability. The CRP immunosensor was developed by covalently conjugating CRP antibodies with 3-mercaptopropionic acid (MPA) on the 3DOM gold film electrode. The CRP concentration was measured through the increase of impedance values in the corresponding specific binding of CRP antigen and CRP antibody. The increased electron-transfer resistance (R-et) values were proportional to the logarithmic value of CRP concentrations in the range of 0.1 to 20 ng mL(-1). The detection of CRP levels in three sera obtained from hospital showed acceptable accuracy.