Quantification of intracellular amino acids in batch cultures of Saccharomyces cerevisiae
Quantification of intracellular amino acids in batch cultures of Saccharomyces cerevisiae
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DOI：
10.1007/s002530100708
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发表时间：
2001-09-01
影响因子：
5
通讯作者：
Wittmann, C
中科院分区：
文献类型：
--
作者：
Hans, MA;Heinzle, E;Wittmann, C
研究了酵母胞内氨基酸池的动态变化。由于在提取过程中观察到的大多数细胞内氨基酸池的显著变化而没有代谢的瞬时停止，因此发现代谢活动的立即终止对于准确定量细胞内氨基酸的体内浓度是必要的。该方法应用于酵母在葡萄糖上的批量培养，揭示了细胞内氨基酸池的复杂动态。最剧烈的变化是在双氧移和进入固定相时观察到的。即使是在葡萄糖和乙醇呈指数增长的阶段。细胞内氨基酸浓度有显著差异。所提出的方法可用于研究酵母培养物的生理，包括工业上相关的批处理和饲料批处理。
The dynamics of intracellular amino acid pools were determined in batch cultures of Saccharomyces cerevisiae. Immediate termination of metabolic activity was found to be necessary for accurate quantification of in vivo concentrations of intracellular amino acids, due to significant changes in most intracellular amino acid pools observed during extraction without an instantaneous stop of the metabolism. The method applied to batch-cultures of S. cerevisiae on glucose revealed complex dynamics in intracellular amino acid pools. The most drastic changes were observed during the diauxic shift and at the entry into the stationary phase. Even during phases of exponential growth on glucose and ethanol. cells showed significant variations in intracellular amino acid concentrations. The method presented can be used to investigate the physiology of yeast cultures, including industrially relevant batch and fed-batch processes.