The Late Effects of Radiation Therapy on Skeletal Muscle Morphology and Progenitor Cell Content are Influenced by Diet-Induced Obesity and Exercise Training in Male Mice
The Late Effects of Radiation Therapy on Skeletal Muscle Morphology and Progenitor Cell Content are Influenced by Diet-Induced Obesity and Exercise Training in Male Mice
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DOI：
10.1038/s41598-019-43204-8
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发表时间：
2019-04-30
影响因子：
4.6
通讯作者：
De Lisio, Michael
中科院分区：
文献类型：
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作者：
D'Souza, Donna;Roubos, Sophia;De Lisio, Michael
肌肉发育期间的辐射暴露会导致骨骼肌健康的长期衰退，从而导致儿童癌症幸存者的生活质量下降。辐射对骨骼肌的影响是否受到相关生理因素的影响，如肥胖和运动训练，目前尚不清楚。利用我们之前发表的研究肥胖和运动训练对辐射暴露骨髓的影响的骨骼肌，我们评估了这些生理宿主因素对辐照骨骼肌形态和细胞动力学的影响。老鼠被分为对照组和高脂肪饮食组，有或没有运动训练。然后，所有小鼠都暴露在辐射中，并在干预组中再持续4周。饮食引起的肥胖导致肌肉纤维化增加，而肥胖和运动训练都增加了肌肉脂肪。运动训练增加了肌纤维的横截面积和用于肌源性谱系的卫星细胞的数量。高脂组p-NF κ B表达增加，IL-6呈下降趋势，TGFB1升高。这些发现表明，与饮食引起的肥胖相比，运动训练可以改善肌肉形态和卫星细胞动力学，并对癌症幸存者的运动干预具有启示意义。
Radiation exposure during muscle development induces long-term decrements to skeletal muscle health, which contribute to reduced quality of life in childhood cancer survivors. Whether the effects of radiation on skeletal muscle are influenced by relevant physiological factors, such as obesity and exercise training remains unknown. Using skeletal muscle from our previously published work examining the effects of obesity and exercise training on radiation-exposed bone marrow, we evaluated the influence of these physiological host factors on irradiated skeletal muscle morphology and cellular dynamics. Mice were divided into control and high fat diet groups with or without exercise training. All mice were then exposed to radiation and continued in their intervention group for an additional 4 weeks. Diet-induced obesity resulted in increased muscle fibrosis, while obesity and exercise training both increased muscle adiposity. Exercise training enhanced myofibre cross-sectional area and the number of satellite cells committed to the myogenic lineage. High fat groups demonstrated an increase in p-NF kappa B expression, a trend for a decline in IL-6, and increase in TGFB1. These findings suggest exercise training improves muscle morphology and satellite cell dynamics compared to diet-induced obesity in irradiated muscle, and have implications for exercise interventions in cancer survivors.