Isolation and Structure Elucidation of Glucosylated Colchicinoids from the Seeds of Gloriosa superba by LC-DAD-SPE-NMR.
Isolation and Structure Elucidation of Glucosylated Colchicinoids from the Seeds of Gloriosa superba by LC-DAD-SPE-NMR.
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DOI：
10.1021/acs.jnatprod.6b01024
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发表时间：
2017-02
影响因子：
5.1
通讯作者：
Y. Zarev;K. Foubert;I. Ionkova;S. Apers;L. Pieters
中科院分区：
文献类型：
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作者：
Y. Zarev;K. Foubert;I. Ionkova;S. Apers;L. Pieters
通过常规柱色谱和 LC-DAD-SPE-NMR，从嘉兰花种子中分离出 4 种新的秋水仙素以及已知化合物秋水仙苷 (4) 和 3-de-O-甲基秋水仙碱-3-O-β-d-吡喃葡萄糖基-(1→4)-3-O-β-d-吡喃葡萄糖苷 (6)。新化合物被鉴定为 N-de乙酰基-N-甲酰基-3-de-O-甲基秋水仙碱-3-O-β-d-吡喃葡萄糖苷 (1)、3-de-O-甲基秋水仙碱-3-O-β-d-吡喃葡萄糖基-(1→6)-3-O-β-d-吡喃葡萄糖苷 (2)、 N-脱乙酰基-N-甲酰基-3-脱-O-甲基秋水仙碱-3-O-β-d-吡喃葡萄糖基-(1→6)-3-O-β-d-吡喃葡萄糖苷(3)和3-脱-O-甲基秋水仙碱-3-O-β-d-吡喃葡萄糖基-(1→3)-3-O-β-d-吡喃葡萄糖苷 （5）。通过NMR（COSY、HSQC和HMBC）、HRESIMS/MS和GCMS数据分析进行结构阐明。
Four new colchicinoids were isolated from the seeds of Gloriosa superba together with the known compounds colchicoside (4) and 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→4)-3-O-β-d-glucopyranoside (6), by means of conventional column chromatography and LC-DAD-SPE-NMR. The new compounds were identified as N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-β-d-glucopyranoside (1), 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→6)-3-O-β-d-glucopyranoside (2), N-deacetyl-N-formyl-3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→6)-3-O-β-d-glucopyranoside (3), and 3-de-O-methylcolchicine-3-O-β-d-glucopyranosyl-(1→3)-3-O-β-d-glucopyranoside (5). The structure elucidation was performed by means of NMR (COSY, HSQC, and HMBC), HRESIMS/MS, and GCMS data analysis.