Suppressor of rid1 (SID1) shares common targets with RID1 on florigen genes to initiate floral transition in rice.
Suppressor of rid1 (SID1) shares common targets with RID1 on florigen genes to initiate floral transition in rice.
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rid1 的抑制因子 (SID1) 与成花素基因上的 RID1 具有共同的靶标，可启动水稻的花转变
DOI：
10.1371/journal.pgen.1006642
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发表时间：
2017-02
期刊：
影响因子：
4.5
通讯作者：
Wu C
中科院分区：
文献类型：
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作者：
Deng L;Li L;Zhang S;Shen J;Li S;Hu S;Peng Q;Xiao J;Wu C
从营养生长到生殖生长的转变是高等植物生活史中的一个关键过程。在此之前，我们克隆了水稻不确定基因1（Rice Indeterminate 1，RID 1），它是水稻从营养生长阶段过渡到生殖生长阶段的主开关。虽然已经建立了RID 1通过Ehd1诱导成花基因Hd3a和RFT1表达的光周期途径，但需要进一步研究基本开花转变的替代途径。在这里，我们确定了一个抑制rid1（SID 1），它挽救了rid1的从不开花表型。SID1编码一个抑制结构域（IDD）转录因子。SID1突变表现为延迟开花表型。SID1、OsIDD1或OsIDD6的功能获得可以使rid1恢复开花。进一步分析表明，SID 1和RID 1直接靶向水稻成花基因Hd3a和RFT1的启动子区。综上所述，我们的研究结果揭示了一个自主开花途径可能是由RID 1介导的，从而控制水稻从营养到生殖发育的阶段转换。
The transition from vegetative to reproductive growth is a critical process in the life cycle of higher plants. Previously, we cloned Rice Indeterminate 1 (RID1), which acts as the master switch for the transition from the vegetative to reproductive phase in rice. Although the photoperiod pathway of RID1 inducing expression of the florigen genes Hd3a and RFT1 via Ehd1 has been established, the alternative pathways for the essential flowering transition need to be further examined. Here, we identified a Suppressor of rid1 (SID1), which rescues the never-flowering phenotype of rid1. SID1 encodes an INDETERMINATE DOMAIN (IDD) transcription factor. Mutation in SID1 showed the delayed flowering phenotype. Gain-of-function of SID1, OsIDD1, or OsIDD6 could restore the rid1 to flowering. Further analyses showed SID1 and RID1 directly target the promoter regions of Hd3a and RFT1, two florigen genes in rice. Taken together, our results reveal an autonomous flowering pathway might be mediated by RID1, thereby controlling the phase transition from vegetative to reproductive development in rice.